ArticleLasers in medical science2012
Effects of low-level laser irradiation on mesenchymal stem cell proliferation: a microarray analysis.
Article in Lasers in medical science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 2 syntheses or guidelines pooled it, 85 citations in OpenAlex.
- Response of osteoblastic cells to low-level laser treatment: a systematic review.Lasers in medical science · 2022Pooled it
- Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review.Lasers in medical science · 2015Pooled it
- Photobiomodulation Meets Mechanotransduction: Immune-Stromal Crosstalk in Orthodontic Remodeling.Biomedicines · 2025Review
- Research progress in different physical therapies for treating peripheral nerve injuries.Frontiers in neurology · 2025Review
- The Use of a Proprietary Near-Infrared Laser to Enhance Wound Healing: A Preliminary Preclinical and Clinical Study.Aesthetic surgery journal. Open forum · 2025Article
- Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs).Scientific reports · 2024Article
- Shedding Light on Photobiomodulation Therapy for Age-Related Macular Degeneration: A Narrative Review.Ophthalmology and therapy · 2023Review
- The Effects and Mechanisms of PBM Therapy in Accelerating Orthodontic Tooth Movement.Biomolecules · 2023Review
- Irradiation with a red light-emitting diode enhances the proliferation of stem cells of apical papilla via the ERK5 signalling pathway.Lasers in medical science · 2022Article
- Outpatient Oral Neuropathic Pain Management with Photobiomodulation Therapy: A Prospective Analgesic Pharmacotherapy-Paralleled Feasibility Trial.Antioxidants (Basel, Switzerland) · 2022Article
- LLLI promotes BMSC proliferation through circRNA_0001052/miR-124-3p.Lasers in medical science · 2022Article
- Combination of Dental Capping Agents With LowLevel Laser Therapy Increases the Cell Viability Percent of Stem Cells From Apical Papilla (SCAPs).Journal of lasers in medical sciences · 2022Article
- Photobiomodulation Regulation as One Promising Therapeutic Approach for Myocardial Infarction.Oxidative medicine and cellular longevity · 2021Review
- In Vitro Cytological Responses against Laser Photobiomodulation for Periodontal Regeneration.International journal of molecular sciences · 2020Review
- Low-level laser irradiation modulates the proliferation and the osteogenic differentiation of bone marrow mesenchymal stem cells under healthy and inflammatory condition.Lasers in medical science · 2019Article
- Photobiomodulation with single and combination laser wavelengths on bone marrow mesenchymal stem cells: proliferation and differentiation to bone or cartilage.Lasers in medical science · 2019Article
- The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review.Journal of lasers in medical sciences · 2019Review
- PTPN21 Overexpression Promotes Osteogenic and Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells but Inhibits the Immunosuppressive Function.Stem cells international · 2019Article
- Low-level laser irradiation induces a transcriptional myotube-like profile in C2C12 myoblasts.Lasers in medical science · 2018Article
- Red (635 nm), Near-Infrared (808 nm) and Violet-Blue (405 nm) Photobiomodulation Potentiality on Human Osteoblasts and Mesenchymal Stromal Cells: A Morphological and Molecular In Vitro Study.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increased proliferation after low-level laser irradiation (LLLI) has been well demonstrated in many cell types including mesenchymal stem cells (MSCs), but the exact molecular mechanisms involved remain poorly understood. The aim of this study was to investigate the change in mRNA expression in rat MSCs after LLLI and to reveal the associated molecular mechanisms. MSCs were exposed to a diode laser (635 nm) as the irradiated group. Cells undergoing the same procedure without LLLI served as the control group. Proliferation was evaluated using the MTS assay. Differences in the gene expression profiles between irradiated and control MSCs at 4 days after LLLI were analyzed using a cDNA microarray. Gene ontology and pathway analysis were used to find the key regulating genes followed by real-time PCR to validate seven representative genes from the microarray assays. This procedure identified 119 differentially expressed genes. Real-time PCR confirmed that the expression levels of v-akt murine thymoma viral oncogene homolog 1 (Akt1), the cyclin D1 gene (Ccnd1) and the phosphatidylinositol 3-kinase, catalytic alpha polypeptide gene (Pik3ca) were upregulated after LLLI, whereas those of protein tyrosine phosphatase non-receptor type 6 (Ptpn6) and serine/threonine kinase 17b (Stk17b) were downregulated. cDNA microarray analysis revealed that after LLLI the expression levels of various genes involved in cell proliferation, apoptosis and the cell cycle were affected. Five genes, including Akt1, Ptpn6, Stk17b, Ccnd1 and Pik3ca, were confirmed and the PI3K/Akt/mTOR/eIF4E pathway was identified as possibly playing an important role in mediating the effects of LLLI on the proliferation of MSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.