ArticleBiomaterials2012
3D cell entrapment in crosslinked thiolated gelatin-poly(ethylene glycol) diacrylate hydrogels.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Recapitulation of human 3D colorectal cancer model using smart thermo-responsive hydrogel for monitoring angiogenesis.Cancer cell international · 2026Article
- Genetic and bioactive functionalization of bioinks for 3D bioprinting.Bioprocess and biosystems engineering · 2025Review
- Design and Characterization of Hybrid Gelatin/PEGDA Hydrogels with Tunable Viscoelastic Properties.Biomacromolecules · 2025Article
- Engineered Living Systems Based on Gelatin: Design, Manufacturing, and Applications.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Injectable functionalised Lycium barbarum polysaccharide/alginate hydrogel particles for the treatment of bone defects.Materials today. Bio · 2025Article
- Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions.Advanced healthcare materials · 2023Review
- Mechanical Evaluation of Hydrogel-Elastomer Interfaces Generated through Thiol-Ene Coupling.ACS applied polymer materials · 2023Article
- Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels forACS applied polymer materials · 2023Article
- Semi-Synthetic Click-Gelatin Hydrogels as Tunable Platforms for 3D Cancer Cell Culture.Gels (Basel, Switzerland) · 2022Article
- Design and Synthesis of Hybrid Thermo-Responsive Hydrogels Based on Poly(2-oxazoline) and Gelatin Derivatives.Gels (Basel, Switzerland) · 2022Article
- Development of cisplatin-loaded hydrogels for trans-portal vein chemoembolization in an orthotopic liver cancer mouse model.Drug delivery · 2021Article
- Modeled vascular microenvironments: immune-endothelial cell interactions in vitro.Drug delivery and translational research · 2021Review
- Norbornene-functionalized methylcellulose as a thermo- and photo-responsive bioink.Biofabrication · 2021Article
- Article
- Development of a Flow-free Gradient Generator Using a Self-Adhesive Thiol-acrylate Microfluidic Resin/Hydrogel (TAMR/H) Hybrid System.ACS applied materials & interfaces · 2021Article
- An injectable micelle-hydrogel hybrid for localized and prolonged drug delivery in the management of renal fibrosis.Acta pharmaceutica Sinica. B · 2021Article
- Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications.Stem cells international · 2021Review
- Thiolation of Biopolymers for Developing Drug Delivery Systems with Enhanced Mechanical and Mucoadhesive Properties: A Review.Polymers · 2020Review
- Three-Dimensional Bioprinting of Perfusable Hierarchical Microchannels with Alginate and Silk Fibroin Double Cross-linked Network.3D printing and additive manufacturing · 2020Article
- Inclusion of Cross-Linked Elastin in Gelatin/PEG Hydrogels Favourably Influences Fibroblast Phenotype.Polymers · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The combined use of natural ECM components and synthetic materials offers an attractive alternative to fabricate hydrogel-based tissue engineering scaffolds to study cell-matrix interactions in three-dimensions (3D). A facile method was developed to modify gelatin with cysteine via a bifunctional PEG linker, thus introducing free thiol groups to gelatin chains. A covalently crosslinked gelatin hydrogel was fabricated using thiolated gelatin and poly(ethylene glycol) diacrylate (PEGdA) via thiol-ene reaction. Unmodified gelatin was physically incorporated in a PEGdA-only matrix for comparison. We sought to understand the effect of crosslinking modality on hydrogel physicochemical properties and the impact on 3D cell entrapment. Compared to physically incorporated gelatin hydrogels, covalently crosslinked gelatin hydrogels displayed higher maximum weight swelling ratio (Q(max)), higher water content, significantly lower cumulative gelatin dissolution up to 7 days, and lower gel stiffness. Furthermore, fibroblasts encapsulated within covalently crosslinked gelatin hydrogels showed extensive cytoplasmic spreading and the formation of cellular networks over 28 days. In contrast, fibroblasts encapsulated in the physically incorporated gelatin hydrogels remained spheroidal. Hence, crosslinking ECM protein with synthetic matrix creates a stable scaffold with tunable mechanical properties and with long-term cell anchorage points, thus supporting cell attachment and growth in the 3D environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.