ArticleRNA biology
Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression.
Article in RNA biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Synergy of RNA Concentration, RNA Binding Proteins, and RNA Palindrome Drives clustering ofbioRxiv : the preprint server for biology · 2026Article
- The mRNA dynamics underpinning translational control mechanisms of Drosophila melanogaster oogenesis.Biochemical Society transactions · 2024Review
- Widespread regulation of the maternal transcriptome by Nanos in Drosophila.PLoS biology · 2024Article
- 3'UTR of mRNA Encoding CPEB Protein Orb2 Plays an Essential Role in Intracellular Transport in Neurons.Cells · 2023Article
- Cup is essential forRNA biology · 2023Article
- Different roles for the adjoining and structurally similar A-rich and poly(A) domains of oskar mRNA: Only the A-rich domain is required for oskar noncoding RNA function, which includes MTOC positioning.Developmental biology · 2021Article
- Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation.PLoS genetics · 2020Article
- No significant regulation of bicoid mRNA by Pumilio or Nanos in the early Drosophila embryo.PloS one · 2018Article
- Review
- MultipleJournal of cell science · 2017Article
- C. elegans SUP-46, an HNRNPM family RNA-binding protein that prevents paternally-mediated epigenetic sterility.BMC biology · 2017Article
- Community effects in regulation of translation.eLife · 2016Article
- Localized Translation of gurken/TGF-α mRNA during Axis Specification Is Controlled by Access to Orb/CPEB on Processing Bodies.Cell reports · 2016Article
- RNA sequences required for the noncoding function of oskar RNA also mediate regulation of Oskar protein expression by Bicoid Stability Factor.Developmental biology · 2015Article
- oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.RNA (New York, N.Y.) · 2015Article
- Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression.PLoS genetics · 2015Article
- CELFish ways to modulate mRNA decay.Biochimica et biophysica actaReview
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4 authors.
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Abstract
Bruno protein binds to multiple sites - BREs - in the oskar mRNA 3' UTR, thereby controlling oskar mRNA translation. Bruno also binds and regulates other mRNAs, although the binding sites have not yet been defined. Bruno has three RRM type RNA binding motifs, two near the amino terminus and an extended RRM at the C terminus. Two domains of Bruno, the first two RRMs (RRM1+2), and the extended RRM (RRM3+) - can each bind with specificity to the oskar mRNA regulatory regions; these and Bruno were used for in vitro selections. Anti-RRM3+ aptamers include long, highly constrained motifs, including one corresponding to the previously identified BRE. Anti-RRM1+2 aptamers lack constrained motifs, but are biased towards classes of short and variable sequences. Bruno itself selects for several motifs, including some of those bound by RRM3+. We propose that the different RNA binding domains allow for combinatorial binding, with extended Bruno binding sites assembled from sequences bound by the individual domains. Examples of such sites were identified in known targets of Bruno, and shown to confer Bruno-dependent translational repression in vivo. Other proteins with multiple RRMs may employ combinatorial binding to achieve high levels of specificity and affinity.
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