ArticleScandinavian journal of immunology2012
FluoroSpot Analysis of TLR-Activated Monocytes Reveals Several Distinct Cytokine-Secreting Subpopulations.
Article in Scandinavian journal of immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Imbalanced Inflammatory Responses in Preterm and Term Cord Blood Monocytes and Expansion of the CD14International journal of molecular sciences · 2023Article
- Longitudinal analysis of DC subsets in patients with ovarian cancer: Implications for immunotherapy.Frontiers in immunology · 2023Article
- Antiphospholipid Antibody Syndrome-Associated Increased Surface Expression of VLA4 Integrin on Human Monocytes.Biomedicines · 2022Article
- Phenotypic and Functional Heterogeneity of Monocyte Subsets in Chronic Heart Failure Patients.Biology · 2022Review
- Toll-like receptor 4 methylation grade is linked to depressive symptom severity.Translational psychiatry · 2021Article
- Selective Recruitment of Monocyte Subsets by Endothelial N-Glycans.The American journal of pathology · 2020Review
- Identification of Novel Human Monocyte Subsets and Evidence for Phenotypic Groups Defined by Interindividual Variations of Expression of Adhesion Molecules.Scientific reports · 2020Article
- Monocyte Subpopulation Recovery as Predictors of Hematopoietic Cell Transplantation Outcomes.Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation · 2019Article
- Role of Monocytes in Heart Failure and Atrial Fibrillation.Journal of the American Heart Association · 2018Review
- Monocyte Subsets: Phenotypes and Function in Tuberculosis Infection.Frontiers in immunology · 2018Review
- New Insights into the Immunobiology of Mononuclear Phagocytic Cells and Their Relevance to the Pathogenesis of Cardiovascular Diseases.Frontiers in immunology · 2017Review
- Association of Canine Osteosarcoma and Monocyte Phenotype and Chemotactic Function.Journal of veterinary internal medicine · 2016Article
- Article
- Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-γ, IL-17A and IL-22 Responses.Cells · 2014Article
- Human monocyte subsets at homeostasis and their perturbation in numbers and function in filarial infection.Infection and immunity · 2014Article
- Clinical validation of IFNγ/IL-10 and IFNγ/IL-2 FluoroSpot assays for the detection of Tr1 T cells and influenza vaccine monitoring in humans.Human vaccines & immunotherapeutics · 2014Article
- Article
- Functional diversity of microglia - how heterogeneous are they to begin with?Frontiers in cellular neuroscience · 2013Article
- Article
- The three human monocyte subsets: implications for health and disease.Immunologic research · 2012Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monocytes have long been considered a heterogeneous group of cells both in terms of morphology and function. In humans, three distinct subsets have been described based on their differential expression of the cell surface markers CD14 and CD16. However, the relationship between these subsets and the production of cytokines has for the most part been based on ELISA measurements, making it difficult to draw conclusions as to their functional profile on the cellular level. In this study, we have investigated lipoteichoic acid (LTA)- and lipopolysaccharide (LPS)-induced cytokine secretion by monocytes using the FluoroSpot technique. This method measures the number of cytokine-secreting cells on the single-cell level and uses fluorescent detection, allowing for the simultaneous analysis of two cytokines from the same population of isolated cells. By this approach, human monocytes from healthy volunteers could be divided into several subgroups as IL-1β, IL-6, TNF-α and MIP-1β were secreted by larger populations of responding cells (25.9-39.2%) compared with the smaller populations of GM-CSF (9.1%), IL-10 (1.3%) and IL-12p40 (1.2%). Furthermore, when studying co-secretion in FluoroSpot, an intricate relationship between the monocytes secreting IL-1β and/or IL-6 and those secreting TNF-α, MIP-1β, GM-CSF, IL-10 and IL-12p40 was revealed. In this way, dissecting the secretion pattern of the monocytes in response to TLR-2 or TLR-4 stimulation, several subpopulations with distinct cytokine-secreting profiles could be identified.
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