ArticleGenetics2011
Dynamics of homology searching during gene conversion in Saccharomyces cerevisiae revealed by donor competition.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Mutations and structural variants arising during double-strand break repair.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Modeling the Homologous Recombination Process: Methods, Successes and Challenges.International journal of molecular sciences · 2023Review
- Highly mismatch-tolerant homology testing by RecA could explain how homology length affects recombination.PloS one · 2023Article
- Cohesin regulates homology search during recombinational DNA repair.Nature cell biology · 2021Article
- Moving forward one step back at a time: reversibility during homologous recombination.Current genetics · 2019Review
- Mating-type switching by homology-directed recombinational repair: a matter of choice.Current genetics · 2019Review
- Dynamic Processing of Displacement Loops during Recombinational DNA Repair.Molecular cell · 2019Article
- Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.Microbial cell (Graz, Austria) · 2019Review
- DNA Repair: The Search for Homology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- ATP hydrolysis provides functions that promote rejection of pairings between different copies of long repeated sequences.Nucleic acids research · 2017Article
- Article
- Homology Requirements and Competition between Gene Conversion and Break-Induced Replication during Double-Strand Break Repair.Molecular cell · 2017Article
- Position effects influencing intrachromosomal repair of a double-strand break in budding yeast.PloS one · 2017Article
- Article
- Chromosome position determines the success of double-strand break repair.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Sgs1 and Exo1 suppress targeted chromosome duplication during ends-in and ends-out gene targeting.DNA repair · 2014Article
- Mechanisms and principles of homology search during recombination.Nature reviews. Molecular cell biology · 2014Review
- Two portable recombination enhancers direct donor choice in fission yeast heterochromatin.PLoS genetics · 2013Article
- Review
- Regulation of budding yeast mating-type switching donor preference by the FHA domain of Fkh1.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
One of the least understood aspects of homologous recombination is the process by which the ends of a double-strand break (DSB) search the entire genome for homologous templates that can be used to repair the break. We took advantage of the natural competition between the alternative donors HML and HMR employed during HO endonuclease-induced switching of the budding yeast MAT locus. The strong mating-type-dependent bias in the choice of the donors is enforced by the recombination enhancer (RE), which lies 17 kb proximal to HML. We investigated factors that improve the use of the disfavored donor. We show that the normal heterochromatic state of the donors does not impair donor usage, as donor choice is not affected by removing this epigenetic silencing. In contrast, increasing the length of homology shared by the disfavored donor increases its use. This result shows that donor choice is not irrevocable and implies that there are several encounters between the DSB ends and even the favored donor before recombination is accomplished. The increase by adding more homology is not linear; these results can be explained by a thermodynamic model that determines the energy cost of using one donor over the other. An important inference from this analysis is that when HML is favored as the donor, RE causes a reduction in its effective genomic distance from MAT from 200 kb to ∼20 kb, which we hypothesize occurs after the DSB is created, by epigenetic chromatin modifications around MAT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.