ArticleGenetics2011
Opaque7 encodes an acyl-activating enzyme-like protein that affects storage protein synthesis in maize endosperm.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 53 papers.
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53 citing papers in PubMed, 89 citations in OpenAlex.
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- Beyond osmoprotection: the expanding roles of proline in plant signalling and development.Biologia futura · 2026Review
- ZmMYB155 is involved in starch synthesis and basal endosperm transfer layer development in maize.Plant cell reports · 2025Article
- ZmSSRP1 facilitates the progression of RNA polymerase II and is essential for kernel development in maize.The Plant cell · 2025Article
- Review
- The maizePlant direct · 2024Article
- ZmDRR206 Regulates Nutrient Accumulation in Endosperm through Its Role in Cell Wall Biogenesis during Maize Kernel Development.International journal of molecular sciences · 2023Article
- A translatome-transcriptome multi-omics gene regulatory network reveals the complicated functional landscape of maize.Genome biology · 2023Article
- A Systemic Investigation of Genetic Architecture and Gene Resources Controlling Kernel Size-Related Traits in Maize.International journal of molecular sciences · 2023Article
- Riboflavin integrates cellular energetics and cell cycle to regulate maize seed development.Plant biotechnology journal · 2022Article
- Combined QTL mapping and RNA-Seq pro-filing reveal candidate genes related to low-temperature tolerance in maize.Molecular breeding : new strategies in plant improvement · 2022Article
- ENB1 encodes a cellulose synthase 5 that directs synthesis of cell wall ingrowths in maize basal endosperm transfer cells.The Plant cell · 2022Article
- Article
- Identification of a new mutant allele ofMolecular breeding : new strategies in plant improvement · 2022Article
- Genetic basis of maize kernel protein content revealed by high-density bin mapping using recombinant inbred lines.Frontiers in plant science · 2022Article
- ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels.The New phytologist · 2021Article
- Trihelix Transcription FactorInternational journal of molecular sciences · 2021Article
- shrunken4 is a mutant allele of ZmYSL2 that affects aleurone development and starch synthesis in maize.Genetics · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Cytosolic malate dehydrogenase 4 modulates cellular energetics and storage reserve accumulation in maize endosperm.Plant biotechnology journal · 2020Article
Corrections and comments
- Retraction · 2022-12-07Duplication of/in Image · Manipulation of Images · Original Data and/or Images not Provided and/or not Available · Unreliable Results and/or Conclusions ·
- Retracted
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In maize, a series of seed mutants with starchy endosperm could increase the lysine content by decreased amount of zeins, the main storage proteins in endosperm. Cloning and characterization of these mutants could reveal regulatory mechanisms for zeins accumulation in maize endosperm. Opaque7 (o7) is a classic maize starchy endosperm mutant with large effects on zeins accumulation and high lysine content. In this study, the O7 gene was cloned by map-based cloning and confirmed by transgenic functional complementation and RNAi. The o7-ref allele has a 12-bp in-frame deletion. The four-amino-acid deletion caused low accumulation of o7 protein in vivo. The O7 gene encodes an acyl-activating enzyme with high similarity to AAE3. The opaque phenotype of the o7 mutant was produced by the reduction of protein body size and number caused by a decrease in the α-zeins concentrations. Analysis of amino acids and metabolites suggested that the O7 gene might affect amino acid biosynthesis by affecting α-ketoglutaric acid and oxaloacetic acid. Transgenic rice seeds containing RNAi constructs targeting the rice ortholog of maize O7 also produced lower amounts of seed proteins and displayed an opaque endosperm phenotype, indicating a conserved biological function of O7 in cereal crops. The cloning of O7 revealed a novel regulatory mechanism for storage protein synthesis and highlighted an effective target for the genetic manipulation of storage protein contents in cereal seeds.
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