ArticleThe Journal of biological chemistry2011
Identification of nicotinamide mononucleotide deamidase of the bacterial pyridine nucleotide cycle reveals a novel broadly conserved amidohydrolase family.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 71 citations in OpenAlex.
- Sublingual NMN administration increases early circulating terminal catabolites 2PY and 4PY compared with oral administration in healthy adult men.Scientific reports · 2026Trial
- Backbone Thioamidation of a Ribosomal Subunit Protein in Pseudomonadota.Biochemistry · 2026Article
- Enhancing cellulase biosynthesis ofiScience · 2025Article
- Structure of a putative terminal amidation domain in natural product biosynthesis.Structure (London, England : 1993) · 2025Article
- Phages reconstitute NADNature · 2024Article
- EngineeringACS catalysis · 2024Article
- Nicotinamide Mononucleotide Supplementation: Understanding Metabolic Variability and Clinical Implications.Metabolites · 2024Review
- Structural insights into Xanthomonas campestris pv. campestris NADCommunications biology · 2024Article
- Enzymology of CaCells · 2023Review
- The NADeLife · 2022Article
- CinA mediates multidrug tolerance in Mycobacterium tuberculosis.Nature communications · 2022Article
- A new family of globally distributed lytic roseophages with unusual deoxythymidine to deoxyuridine substitution.Current biology : CB · 2021Article
- Characterization of Two NMN Deamidase Mutants as Possible Probes for an NMN Biosensor.International journal of molecular sciences · 2021Article
- Age Associated Microbiome and Microbial Metabolites Modulation and Its Association With Systemic Inflammation in a Rhesus Macaque Model.Frontiers in immunology · 2021Article
- Integration of the pSLT Plasmid into theJournal of bacteriology · 2020Article
- Article
- Metabolomics Study of the Synergistic Killing of Polymyxin B in Combination with Amikacin against Polymyxin-Susceptible and -Resistant Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2019Article
- Modular engineering to increase intracellular NAD(H/Nature communications · 2018Article
- Complete Genome Sequence ofGenome announcements · 2017Article
- Underlying mechanisms for syntrophic metabolism of essential enzyme cofactors in microbial communities.The ISME journal · 2017Article
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11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pyridine nucleotide cycle is a network of salvage and recycling routes maintaining homeostasis of NAD(P) cofactor pool in the cell. Nicotinamide mononucleotide (NMN) deamidase (EC 3.5.1.42), one of the key enzymes of the bacterial pyridine nucleotide cycle, was originally described in Enterobacteria, but the corresponding gene eluded identification for over 30 years. A genomics-based reconstruction of NAD metabolism across hundreds of bacterial species suggested that NMN deamidase reaction is the only possible way of nicotinamide salvage in the marine bacterium Shewanella oneidensis. This prediction was verified via purification of native NMN deamidase from S. oneidensis followed by the identification of the respective gene, termed pncC. Enzymatic characterization of the PncC protein, as well as phenotype analysis of deletion mutants, confirmed its proposed biochemical and physiological function in S. oneidensis. Of the three PncC homologs present in Escherichia coli, NMN deamidase activity was confirmed only for the recombinant purified product of the ygaD gene. A comparative analysis at the level of sequence and three-dimensional structure, which is available for one of the PncC family member, shows no homology with any previously described amidohydrolases. Multiple alignment analysis of functional and nonfunctional PncC homologs, together with NMN docking experiments, allowed us to tentatively identify the active site area and conserved residues therein. An observed broad phylogenomic distribution of predicted functional PncCs in the bacterial kingdom is consistent with a possible role in detoxification of NMN, resulting from NAD utilization by DNA ligase.
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