ArticleJournal of gastroenterology2012
Differential effects of nutritional and non-nutritional therapies on intestinal barrier function in an in vitro model.
Article in Journal of gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 63 citations in OpenAlex.
- The efficacy of dietary therapies in modulating inflammatory biomarkers, clinical remission and quality of life in patients with inflammatory bowel disease: a network meta-analysis of 15 interventions.Frontiers in nutrition · 2025Pooled it
- Enteral Nutrition in Patients with Inflammatory Bowel Disease. Systematic Review, Meta-Analysis, and Meta-Regression.Nutrients · 2019Pooled it
- Mechanisms of Action of Exclusive Enteral Nutrition and Other Nutritional Therapies in Crohn's Disease.Nutrients · 2024Review
- Exclusive Enteral Nutrition Beneficially Modulates Gut Microbiome in a Preclinical Model of Crohn's-like Colitis.Nutrients · 2024Article
- Inflammatory cytokines directly disrupt the bovine intestinal epithelial barrier.Scientific reports · 2022Article
- A Preliminary Study of Modulen IBD Liquid Diet in Hospitalized Dogs with Protein-Losing Enteropathy.Animals : an open access journal from MDPI · 2022Article
- Manipulation of Gut Microbiota as a Key Target for Crohn's Disease.Frontiers in medicine · 2022Review
- Review of exclusive enteral therapy in adult Crohn's disease.BMJ open gastroenterology · 2021Review
- Nutritional Aspects of Pediatric Gastrointestinal Diseases.Nutrients · 2021Review
- Dietary Management in Pediatric Patients with Crohn's Disease.Nutrients · 2021Review
- Metabolomic Signatures in Pediatric Crohn's Disease Patients with Mild or Quiescent Disease Treated with Partial Enteral Nutrition: A Feasibility Study.SLAS technology · 2021Article
- Nutritional Therapy Strategies in Pediatric Crohn's Disease.Nutrients · 2021Review
- Exclusive Enteral Nutrition in Adult Crohn's Disease: an Overview of Clinical Practice and Perceived Barriers.Clinical and experimental gastroenterology · 2021Review
- Diet and Nutrients in Gastrointestinal Chronic Diseases.Nutrients · 2020Review
- Review
- Towards a Food Pharmacy: Immunologic Modulation through Diet.Nutrients · 2019Review
- An overview of the role of exclusive enteral nutrition for complicated Crohn's disease.Intestinal research · 2019Article
- Recipe for IBD: can we use food to control inflammatory bowel disease?Seminars in immunopathology · 2018Review
- The impact of exclusive enteral nutrition on the intestinal microbiota in inflammatory bowel disease.AIMS microbiology · 2018Review
- The major pathway by which polymeric formula reduces inflammation in intestinal epithelial cells: a microarray-based analysis.Genes & nutrition · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDiminished intestinal epithelial barrier function contributes to the pathogenesis of Crohn's disease. Clinical and experimental studies propose that increased tumor necrosis factor (TNF)-α promotes barrier dysfunction. The aim of this study was to investigate the effects of nutritional and other therapies upon intestinal barrier function in the presence of TNF-α in an in vitro model.
methodsCaco-2 monolayers were grown to confluence on membrane supports and then exposed to TNF-α in the presence of polymeric formula, hydrocortisone or infliximab. Monolayer permeability was evaluated by measuring epithelial resistance, short-circuit current and horseradish peroxidase flux in an Ussing chamber. Tight junction and myosin II regulatory light-chain kinase gene expression was analysed by real-time PCR, with protein expression and localization analysed by Western blot and immunofluorescence.
resultsTNF-α increased monolayer permeability and diminished tight junction integrity. However both polymeric formula and infliximab completely abrogated the effects of TNF-α. These monolayers displayed unchanged permeability and tight junction integrity compared to untreated cells (media-no-TNF-α controls). In contrast, hydrocortisone only partially abrogated the effects of TNF-α, with these monolayers having increased permeability and altered tight junction integrity compared to media-no-TNF-α controls.
conclusionsBoth polymeric formula and infliximab completely prevent epithelial barrier dysfunction in the presence of TNF-α, whereas hydrocortisone partially prevents barrier dysfunction. These results provide evidence that superior mucosal healing can be achieved with both polymeric formula and infliximab compared to hydrocortisone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.