ArticleJournal of the American Society for Mass Spectrometry2011
Diagnosing the protonation site of b2 peptide fragment ions using IRMPD in the X-H (X = O, N, and C) stretching region.
Article in Journal of the American Society for Mass Spectrometry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 46 citations in OpenAlex.
- Structure and fragmentation chemistry of the peptide radical cations of glycylphenylalanylglycine (GFG).PloS one · 2024Article
- Participation of C-H Protons in the Dissociation of a Proton Deficient Dipeptide.Journal of the American Society for Mass Spectrometry · 2017Article
- Combined Liquid Chromatography-Infrared Ion Spectroscopy for Identification of Regioisomeric Drug Metabolites.Analytical chemistry · 2017Article
- Deamidation Reactions of Asparagine- and Glutamine-Containing Dipeptides Investigated by Ion Spectroscopy.Journal of the American Society for Mass Spectrometry · 2016Article
- Investigation of bn-44 peptide fragments using high resolution mass spectrometry and isotope labeling.Journal of the American Society for Mass Spectrometry · 2014Article
- Zundel-type H-bonding in biomolecular ions.Journal of the American Society for Mass Spectrometry · 2014Article
- Spectroscopic identification of cyclic imide b2-ions from peptides containing Gln and Asn residues.Journal of the American Society for Mass Spectrometry · 2013Article
- Conformation-specific spectroscopy of peptide fragment ions in a low-temperature ion trap.Journal of the American Society for Mass Spectrometry · 2012Article
- Rearrangement pathways of the a (4) ion of protonated YGGFL characterized by IR spectroscopy and modeling.Journal of the American Society for Mass Spectrometry · 2012Article
- Calculations of relative intensities of fragment ions in the MSMS spectra of a doubly charged penta-peptide.BMC bioinformatics · 2012Article
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Charge-directed fragmentation has been shown to be the prevalent dissociation step for protonated peptides under the low-energy activation (eV) regime. Thus, the determination of the ion structure and, in particular, the characterization of the protonation site(s) of peptides and their fragments is a key approach to substantiate and refine peptide fragmentation mechanisms. Here we report on the characterization of the protonation site of oxazolone b(2) ions formed in collision-induced dissociation (CID) of the doubly protonated tryptic model-peptide YIGSR. In support of earlier work, here we provide complementary IR spectra in the 2800-3800 cm(-1) range acquired on a table-top laser system. Combining this tunable laser with a high power CO(2) laser to improve spectroscopic sensitivity, well resolved bands are observed, with an excellent correspondence to the IR absorption bands of the ring-protonated oxazolone isomer as predicted by quantum chemical calculations. In particular, it is shown that a band at 3445 cm(-1), corresponding to the asymmetric N-H stretch of the (nonprotonated) N-terminal NH(2) group, is a distinct vibrational signature of the ring-protonated oxazolone structure.
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