ArticleJournal of the American Society for Mass Spectrometry2011
LC-MS(n) analysis of isomeric chondroitin sulfate oligosaccharides using a chemical derivatization strategy.
Article in Journal of the American Society for Mass Spectrometry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- Mass Spectrometric Determination of Site-SpecificJournal of the American Society for Mass Spectrometry · 2026Article
- Glycosaminoglycans as Polyelectrolytes: Charge, Interactions, and Applications.Chembiochem : a European journal of chemical biology · 2025Review
- Mass Spectrometry Approach for Differentiation of Positional Isomers of Saccharides: Toward Direct Analysis of Rare Sugars.Analytical chemistry · 2023Article
- Straightforward Analysis of Sulfated Glycosaminoglycans by MALDI-TOF Mass Spectrometry from Biological Samples.Biology · 2022Article
- Chemical Modification of Glycosaminoglycan Polysaccharides.Molecules (Basel, Switzerland) · 2021Review
- Effective Inhibition of SARS-CoV-2 Entry by Heparin and Enoxaparin Derivatives.Journal of virology · 2021Article
- Mimicking Extracellular Matrix via Engineered Nanostructured Biomaterials for Neural Repair.Current neuropharmacology · 2021Review
- Peracylation Coupled with Tandem Mass Spectrometry for Structural Sequencing of Sulfated Glycosaminoglycan Mixtures without Depolymerization.Journal of the American Society for Mass Spectrometry · 2020Article
- Effective Inhibition of SARS-CoV-2 Entry by Heparin and Enoxaparin Derivatives.bioRxiv : the preprint server for biology · 2020Article
- Salt-free fractionation of complex isomeric mixtures of glycosaminoglycan oligosaccharides compatible with ESI-MS and microarray analysis.Scientific reports · 2019Article
- Structural Characterization of Glycosaminoglycan Carbohydrates Using Ultraviolet Photodissociation.Analytical chemistry · 2019Article
- An Automated, High-Throughput Method for Interpreting the Tandem Mass Spectra of Glycosaminoglycans.Journal of the American Society for Mass Spectrometry · 2018Article
- Analysis of Sulfate Patterns in Glycosaminoglycan Oligosaccharides by MSJournal of the American Society for Mass Spectrometry · 2018Article
- Structural elucidation of fucosylated chondroitin sulfates from sea cucumber using FTICR-MS/MS.European journal of mass spectrometry (Chichester, England) · 2018Article
- Single Stage Tandem Mass Spectrometry Assignment of the C-5 Uronic Acid Stereochemistry in Heparan Sulfate Tetrasaccharides using Electron Detachment Dissociation.Journal of the American Society for Mass Spectrometry · 2017Article
- De Novo Sequencing of Complex Mixtures of Heparan Sulfate Oligosaccharides.Analytical chemistry · 2016Article
- Assignment Of Hexuronic Acid Stereochemistry In Synthetic Heparan Sulfate Tetrasaccharides With 2-International journal of mass spectrometry · 2015Article
- Oligosaccharide analysis by mass spectrometry: a review of recent developments.Analytical chemistry · 2014Review
- Mass spectral profiling of glycosaminoglycans from histological tissue surfaces.Analytical chemistry · 2013Article
- An approach for separation and complete structural sequencing of heparin/heparan sulfate-like oligosaccharides.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Improved methods for structural analyses of glycosaminoglycans (GAGs) are required to understand their functional roles in various biological processes. Major challenges in structural characterization of complex GAG oligosaccharides using liquid chromatography-mass spectrometry (LC-MS) include the accurate determination of the patterns of sulfation due to gas-phase losses of the sulfate groups upon collisional activation and inefficient on-line separation of positional sulfation isomers prior to MS/MS analyses. Here, a sequential chemical derivatization procedure including permethylation, desulfation, and acetylation was demonstrated to enable both on-line LC separation of isomeric mixtures of chondroitin sulfate (CS) oligosaccharides and accurate determination of sites of sulfation by MS(n). The derivatized oligosaccharides have sulfate groups replaced with acetyl groups, which are sufficiently stable to survive MS(n) fragmentation and reflect the original sulfation patterns. A standard reversed-phase LC-MS system with a capillary C18 column was used for separation, and MS(n) experiments using collision-induced dissociation (CID) were performed. Our results indicate that the combination of this derivatization strategy and MS(n) methodology enables accurate identification of the sulfation isomers of CS hexasaccharides with either saturated or unsaturated nonreducing ends. Moreover, derivatized CS hexasaccharide isomer mixtures become separable by LC-MS method due to different positions of acetyl modifications.
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