ArticlePflugers Archiv : European journal of physiology2012
Regulation of Na+/H+ exchanger in dendritic cells by Akt2.
Article in Pflugers Archiv : European journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 9 citations in OpenAlex.
- Akt isoforms in the immune system.Frontiers in immunology · 2022Review
- Lumen LPS inhibits HCO3(-) absorption in the medullary thick ascending limb through TLR4-PI3K-Akt-mTOR-dependent inhibition of basolateral Na+/H+ exchange.American journal of physiology. Renal physiology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dendritic cells (DCs) are antigen-presenting cells decisive in primary immune responses and establishment of immunological memory. They are activated by bacterial lipopolysaccharides (LPS), which lead to activation of Na(+)/H(+) exchanger activity, cell swelling, reactive oxygen species (ROS) formation, and migration. The effects require functional phosphoinositide 3 kinase and are paralleled by Akt phosphorylation. The present study explored the putative involvement of the Akt isoform Akt2. To this end, experiments were performed in DCs isolated from bone marrow of mice lacking functional Akt2/PKBß (akt2 (-/-)) and respective wild-type animals (akt2 (+/+)). Based on BCECF fluorescence, cytosolic pH (pH(i)) was significantly lower in akt2 (-/-) than in akt2 (+/+) DCs. Transient exposure to NH(4)Cl was followed by profound cytosolic acidification in both genotypes. Subsequent re-alkalinization was largely dependent on Na(+) thus reflecting Na(+)/H(+) exchanger activity and was significantly lower in akt2 (-/-) than in akt2 (+/+) DCs. According to forward scatter in FACS analysis, cell volume was significantly lower in akt2 (-/-) than in akt2 (+/+) DCs. Exposure of DCs to LPS led within 4 h to significant increases of Na(+)/H(+) exchanger activity, cell volume, ROS production, and migration in akt2 (+/+) mice, and its effects were significantly blunted in akt2 (-/-) DCs. The present observations disclose a role of Akt2 in the regulation of pH(i), cell volume, ROS production, and migration in dendritic cells.
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