ArticleJournal of the American Society for Mass Spectrometry2011
An electrostatic charge partitioning model for the dissociation of protein complexes in the gas phase.
Article in Journal of the American Society for Mass Spectrometry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Ribosome Heterogeneity Revealed by Complex-Up Native Mass Spectrometry and Top-Down Proteomics.Journal of the American Chemical Society · 2026Article
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- Biofunctionalized dissolvable hydrogel microbeads enable efficient characterization of native protein complexes.Nature communications · 2024Article
- Fe protein docking transduces conformational changes to MoFe nitrogenase active site in a nucleotide-dependent manner.Communications chemistry · 2023Article
- Immunocomplexed Antigen Capture and Identification by Native Top-Down Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2023Article
- Combining Surface-Induced Dissociation and Charge Detection Mass Spectrometry to Reveal the Native Topology of Heterogeneous Protein Complexes.Analytical chemistry · 2023Article
- Gas-Phase Unfolding of Protein Complexes Distinguishes Conformational Isomers.Journal of the American Chemical Society · 2022Article
- High-Resolution Native Mass Spectrometry.Chemical reviews · 2022Review
- Surface-induced Dissociation Mass Spectrometry as a Structural Biology Tool.Chemical reviews · 2022Review
- The challenge of structural heterogeneity in the native mass spectrometry studies of the SARS-CoV-2 spike protein interactions with its host cell-surface receptor.Analytical and bioanalytical chemistry · 2021Article
- Mass Spectrometric and Bio-Computational Binding Strength Analysis of Multiply Charged RNAse S Gas-Phase Complexes Obtained by Electrospray Ionization from Varying In-Solution Equilibrium Conditions.International journal of molecular sciences · 2021Article
- Surface-Induced Dissociation of Anionic vs Cationic Native-Like Protein Complexes.Journal of the American Chemical Society · 2021Article
- Platelet Factor 4 Interactions with Short Heparin Oligomers: Implications for Folding and Assembly.Biophysical journal · 2020Article
- Ultraviolet Photodissociation Mass Spectrometry for Analysis of Biological Molecules.Chemical reviews · 2020Review
- Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE).Molecular & cellular proteomics : MCP · 2019Article
- Impact of charge state on 193 nm ultraviolet photodissociation of protein complexes.Physical chemistry chemical physics : PCCP · 2019Article
- Experimental and theoretical investigation of overall energy deposition in surface-induced unfolding of protein ions.Chemical science · 2019Article
- Aryl bis-sulfonamides bind to the active site of a homotrimeric isoprenoid biosynthesis enzyme IspF and extract the essential divalent metal cation cofactor.Chemical science · 2018Article
- The Role of Mass Spectrometry in Structural Studies of Flavin-Based Electron Bifurcating Enzymes.Frontiers in microbiology · 2018Review
- Native Electron Capture Dissociation Maps to Iron-Binding Channels in Horse Spleen Ferritin.Analytical chemistry · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrosprayed multi-protein complexes can be dissociated by collisional activation in the gas phase. Typically, these processes follow a mechanism whereby a single subunit gets ejected with a disproportionately high amount of charge relative to its mass. This asymmetric behavior suggests that the departing subunit undergoes some degree of unfolding prior to being separated from the residual complex. These structural changes occur concomitantly with charge (proton) transfer towards the subunit that is being unraveled. Charge accumulation takes place up to the point where the subunit loses physical contact with the residual complex. This work develops a simple electrostatic model for studying the relationship between conformational changes and charge enrichment during collisional activation. Folded subunits are described as spheres that carry continuum surface charge. The unfolded chain is envisioned as random coil bead string. Simulations are guided by the principle that the system will adopt the charge configuration with the lowest potential energy for any backbone conformation. A finite-difference gradient algorithm is used to determine the charge on each subunit throughout the dissociation process. Both dimeric and tetrameric protein complexes are investigated. The model reproduces the occurrence of asymmetric charge partitioning for dissociation events that are preceded by subunit unfolding. Quantitative comparisons of experimental MS/MS data with model predictions yield estimates of the structural changes that occur during collisional activation. Our findings suggest that subunit separation can occur over a wide range of scission point structures that correspond to different degrees of unfolding.
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