ArticleBritish journal of cancer2011
PTEN regulates colorectal epithelial apoptosis through Cdc42 signalling.
Article in British journal of cancer, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Molecular docking, molecular dynamics simulations andRSC advances · 2023Article
- Review
- Cloning and Differential Expression Analyses of Cdc42 from Sheep.Journal of veterinary research · 2018Article
- PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.eLife · 2017Article
- Reduced beta 2 glycoprotein I prevents high glucose-induced cell death in HUVECs through miR-21/PTEN.American journal of translational research · 2017Article
- Vitamin D3 suppresses morphological evolution of the cribriform cancerous phenotype.Oncotarget · 2016Article
- Association of genetic polymorphisms in PTEN and additional interaction with alcohol consumption and smoking on colorectal cancer in Chinese population.International journal of clinical and experimental medicine · 2015Article
- A dynamic exchange of TCF3 and TCF4 transcription factors controls MYC expression in colorectal cancer cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN.World journal of gastroenterology · 2013Article
- PTEN phosphatase-independent maintenance of glandular morphology in a predictive colorectal cancer model system.Neoplasia (New York, N.Y.) · 2013Article
- Rescue of glandular dysmorphogenesis in PTEN-deficient colorectal cancer epithelium by PPARγ-targeted therapy.Oncogene · 2013Article
- PTEN regulation of ERK1/2 signaling in cancer.Journal of receptor and signal transduction research · 2012Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPhosphatase and tensin homologue deleted on chromosome 10 (PTEN) regulation of the Rho-like GTPase Cdc42 has a central role in epithelial polarised growth, but effects of this molecular network on apoptosis remain unclear.
methodsTo investigate the role of Cdc42 in PTEN-dependent cell death, we used flow cytometry, in vitro pull-down assays, poly(ADP ribose) polymerase (PARP) cleavage and other immunoblots in isogenic PTEN-expressing and -deficient colorectal cells (HCT116PTEN(+/+), HCT116PTEN(-/-), Caco2 and Caco2 ShPTEN cells) after transfection or treatment strategies.
resultsThe PTEN knockout or suppression by short hairpin RNA or small interfering RNA (siRNA) inhibited Cdc42 activity, PARP cleavage and/or apoptosis in flow cytometry assays. Transfection of cells with wild-type or constitutively active Cdc42 enhanced PARP cleavage, whereas siRNA silencing of Cdc42 inhibited PARP cleavage and/or apoptosis. Pharmacological upregulation of PTEN by sodium butyrate (NaBt) treatment enhanced Cdc42 activity, PARP cleavage and apoptosis, whereas Cdc42 siRNA suppressed NaBt-induced PARP cleavage. Cdc42-dependent signals can suppress glycogen synthase kinase-β (GSK3β) activity. Pharmacological inhibition of GSK3β by lithium chloride treatment mimicked effects of Cdc42 in promotion of PARP cleavage and/or apoptosis.
conclusionPhosphatase and tensin homologue deleted on chromosome 10 may influence apoptosis in colorectal epithelium through Cdc42 signalling, thus providing a regulatory framework for both polarised growth and programmed cell death.
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