ArticleJournal of microbiology and biotechnology2011
Molecular cloning and characterization of mannitol-1-phosphate dehydrogenase from Vibrio cholerae.
Article in Journal of microbiology and biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Abrogating the adenine methylation ability of Lacticaseibacillus paracasei improves its freeze-drying and storage resistance.NPJ science of food · 2025Article
- Membrane Efflux Pumps of PathogenicMicroorganisms · 2022Review
- MtlR negatively regulates mannitol utilization byMicrobiology (Reading, England) · 2017Article
- Comparative genome analysis of non-toxigenic non-O1 versus toxigenic O1Genomics discoveryArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio cholerae utilizes mannitol through an operon of the phosphoenolpyruvate-dependent phosphotransferase (PTS) type. A gene, mtlD, encoding mannitol-1-phosphate dehydrogenase was identified within the 3.9 kb mannitol operon of V. cholerae. The mtlD gene was cloned from V. cholerae O395, and the recombinant enzyme was functionally expressed in E. coli as a 6×His-tagged protein and purified to homogeneity. The recombinant protein is a monomer with a molecular mass of 42.35 kDa. The purified recombinant MtlD reduced fructose 6-phosphate (F6P) using NADH as a cofactor with a K(m) of 1.54 +/- 0.1 mM and V(max) of 320.8 +/- 7.81 micronmol/min/mg protein. The pH and temperature optima for F6P reduction were determined to be 7.5 and 37°C, respectively. Using quantitative real-time PCR analysis, mtlD was found to be constitutively expressed in V. cholerae, but the expression was up-regulated when grown in the presence of mannitol. The MtlD expression levels were not significantly different between V. cholerae O1 and non-O1 strains.
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Registered trials
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