Evidence map›Paper›PMID 21952042›Full record

ArticleExperimental neurology2012

Alterations in chondroitin sulfate proteoglycan expression occur both at and far from the site of spinal contusion injury.

Ellen M Andrews, Rebekah J Richards, Feng Q Yin, Mariano S Viapiano, Lyn B Jakeman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental neurology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed, 1 pooled it
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed, 1 synthesis or guideline pooled it, 101 citations in OpenAlex.

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  5. The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024
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8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ellen M AndrewsDepartment of Physiology and Cell Biology, The Ohio State University Medical Center, Columbus, OH 43210, USA.
Rebekah J Richards
Feng Q Yin
Mariano S Viapiano
Lyn B Jakeman
The Ohio State University · USThe Ohio State University Wexner Medical Center · US

Funding

Axons and the Extracellular Matrix in Spinal Cord InjuryR01NS043246 · NINDS · OHIO STATE UNIVERSITY · PI MCTIGUE, DANA M · 2004 to 2015
$3.0M
Targeting the Tumor Matrix as Anti-Invasive and Sensitizing Strategy for GliomaR01CA152065 · NCI · OHIO STATE UNIVERSITY · PI VIAPIANO, MARIANO SEBASTIAN · 2010 to 2014
$1.3M
Howard Hughes Medical InstituteNCI NIH HHS R01 CA152065NINDS NIH HHS NS43246NINDS NIH HHS R01 NS043246
6 · The paper itself

Abstract

Chondroitin sulfate proteoglycans (CSPGs) present an inhibitory barrier to axonal growth and plasticity after trauma to the central nervous system. These extracellular and membrane bound molecules are altered after spinal cord injuries, but the magnitude, time course, and patterns of expression following contusion injury have not been fully described. Western blots and immunohistochemistry were combined to assess the expression of four classically inhibitory CSPGs, aggrecan, neurocan, brevican and NG2, at the lesion site and in distal segments of cervical and thoracic spinal cord at 3, 7, 14 and 28 days following a severe mid-thoracic spinal contusion. Total neurocan and the full-length (250 kDa) isoform were strongly upregulated both at the lesion epicenter and in cervical and lumbar segments. In contrast, aggrecan and brevican were sharply reduced at the injury site and were unchanged in distal segments. Total NG2 protein was unchanged across the injury site, while NG2+ profiles were distributed throughout the lesion site by 14 days post-injury (dpi). Far from the lesion, NG2 expression was increased at lumbar, but not cervical spinal cord levels. To determine if the robust increase in neurocan at the distal spinal cord levels corresponded to regions of increased astrogliosis, neurocan and GFAP immunoreactivity were measured in gray and white matter regions of the spinal enlargements. GFAP antibodies revealed a transient increase in reactive astrocyte staining in cervical and lumbar cord, peaking at 14 dpi. In contrast, neurocan immunoreactivity was specifically elevated in the cervical dorsal columns and in the lumbar ventral horn and remained high through 28 dpi. The long lasting increase of neurocan in gray matter regions at distal levels of the spinal cord may contribute to the restriction of plasticity in the chronic phase after SCI. Thus, therapies targeted at altering this CSPG both at and far from the lesion site may represent a reasonable addition to combined strategies to improve recovery after SCI.

Indexed as

AnimalsAstrocytesAxonsChondroitin Sulfate ProteoglycansFemaleGlial Fibrillary Acidic ProteinNeuronsRatsRats, Sprague-DawleySpinal CordSpinal Cord InjuriesChondroitin Sulfate ProteoglycansGlial Fibrillary Acidic Protein

Identifiers

PMID21952042
PMCPMC3640493
OpenAlexW2162066460

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.