Evidence map›Paper›PMID 21951527›Full record

ArticleMethods in cell biology2011

Study of host-microbe interactions in zebrafish.

Kathryn Milligan-McClellan, Jeremy R Charette, Ryan T Phennicie, W Zac Stephens, John F Rawls, Karen Guillemin, Carol H Kim

Open access · greenAbstract read
In one paragraph

Article in Methods in cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.

0numbers the graph read from it
0cells of the map it votes in
78citing papers in PubMed
8.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

78 citing papers in PubMed, 127 citations in OpenAlex.

  1. Article
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  8. ThemBio · 2025
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  11. Multiplexed In Vivo Imaging with Fluorescence Lifetime-Modulating Tags.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
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  15. Who's talking to whom: microbiome-enteric nervous system interactions in early life.American journal of physiology. Gastrointestinal and liver physiology · 2023
    Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

18 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kathryn Milligan-McClellanInstitute of Molecular Biology, University of Oregon, Eugene, Oregon, USA.
Jeremy R Charette
Ryan T Phennicie
W Zac Stephens
John F Rawls
Karen Guillemin
Carol H Kim
University of Maine · USUniversity of Oregon · USUniversity of North Carolina at Chapel Hill · US

Funding

Quantitative Biology Research InstituteP20GM103534 · NIGMS · DARTMOUTH COLLEGE · PI AMOS, CHRISTOPHER I. · 2012 to 2016
$8.6M
Microbial Regulation of Host Nutrient MetabolismR01DK081426 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI RAWLS, JOHN F · 2008 to 2019
$3.9M
Functional Role of Zebrafish TLR ProteinsR01GM087308 · NIGMS · UNIVERSITY OF MAINE ORONO · PI KIM, CAROL H. · 2008 to 2012
$1.5M
Host genetic regulation of intestinal microbial community membershipF32DK096753 · NIDDK · UNIVERSITY OF OREGON · PI MILLIGAN-MCCLELLAN, KATHRYN C.A. · 2012 to 2014
$163k
NIDDK NIH HHS F32 DK096753NIDDK NIH HHS R01 DK081426NIGMS NIH HHS P20 GM103534NIGMS NIH HHS R01 GM087308
6 · The paper itself

Abstract

All animals are ecosystems, home to diverse microbial populations. Animal-associated microbes play important roles in the normal development and physiology of their hosts, but can also be agents of infectious disease. Traditionally, mice have been used to study pathogenic and beneficial associations between microbes and vertebrate animals. The zebrafish is emerging as a valuable new model system for host-microbe interaction studies, affording researchers with the opportunity to survey large populations of hosts and to visualize microbe-host associations at a cellular level in living animals. This chapter provides detailed protocols for the analysis of zebrafish-associated microbial communities, the derivation and husbandry of germ-free zebrafish, and the modeling of infectious disease in different stages of zebrafish development via different routes of inoculation. These protocols offer a starting point for researchers to address a multitude of questions about animals' coexistence with microorganisms.

Indexed as

Bacterial Typing TechniquesGerm-Free LifeHost-Pathogen InteractionsLarvaZebrafishAnimalsBacteriaBacterial InfectionsDevelopmental BiologyDisease Models, AnimalEmbryo, NonmammalianIn Situ Hybridization, FluorescenceIntestinesMiceMicrobial ConsortiaMicrobial InteractionsRNA, BacterialRNA, Ribosomal, 16S

Identifiers

PMID21951527
PMCPMC4700925
OpenAlexW1492464709

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.