ArticleThe Journal of cell biology1990
A test of microtubule translocation during neurite elongation.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed.
- Feedback-Driven Dynamical Model for Axonal Extension on Parallel Micropatterns.Biomimetics (Basel, Switzerland) · 2025Article
- Mathematical models of neuronal growth.Biomechanics and modeling in mechanobiology · 2022Review
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Neurite elongation is highly correlated with bulk forward translocation of microtubules.Scientific reports · 2017Article
- Moonlighting Motors: Kinesin, Dynein, and Cell Polarity.Trends in cell biology · 2017Review
- The emerging role of forces in axonal elongation.Progress in neurobiology · 2011Review
- Phosphorylation of SCG10/stathmin-2 determines multipolar stage exit and neuronal migration rate.Nature neuroscience · 2011Article
- Neurofilaments form a highly stable stationary cytoskeleton after reaching a critical level in axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- A physical model of axonal elongation: force, viscosity, and adhesions govern the mode of outgrowth.Biophysical journal · 2008Article
- Direct evidence for coherent low velocity axonal transport of mitochondria.The Journal of cell biology · 2006Article
- JNK1 phosphorylation of SCG10 determines microtubule dynamics and axodendritic length.The Journal of cell biology · 2006Article
- Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments.The Journal of cell biology · 2005Article
- Live-cell imaging of slow axonal transport in cultured neurons.Methods in cell biology · 2003Article
- Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.Molecular biology of the cell · 2001Article
- Neurofilaments are transported rapidly but intermittently in axons: implications for slow axonal transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Slow axonal transport of neurofilament protein in cultured neurons.The Journal of cell biology · 1999Article
- Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Microtubule transport from the cell body into the axons of growing neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Dynamic microtubule ends are required for growth cone turning to avoid an inhibitory guidance cue.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In a previous study using PC-12 cells (Lim, S. S., P. J. Sammak, and G. G. Borisy, 1989. J. Cell Biol. 109:253-263), we presented evidence that the microtubule component of the neuronal cytoskeleton is differentially dynamic but stationary. However, neurites of PC-12 cells grow slowly, hindering a stringent test of slow axonal transport mechanisms under conditions where growth was substantial. We therefore extended our studies to primary cultures of dorsal root ganglion cells where the rate of neurite outgrowth is rapid. Cells were microinjected with X-rhodamine-labeled tubulin 7-16 h after plating. After a further incubation for 6-18 h, the cells were photobleached with an argon ion laser. Using a cooled charged couple device and video microscopy, the cells were monitored for growth of the neurite and movement and recovery of fluorescence in the bleached zone. As for PC-12 cells, all bleached zones in the neurite recovered their fluorescence, indicating that incorporation of tubulin occurred along the neurite. Despite increases in neurite length of up to 70 microns, and periods of observation of up to 5 h, no movement of bleached zones was observed. We conclude that neurite elongation cannot be accounted for by the transport of a microtubule network assembled only at the cell body. Rather, microtubules turn over all along the length of the neurite and neurite elongation occurs by net assembly at the tip.
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Registered trials
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