ArticlePloS one2011
Evaluation of intra-host variants of the entire hepatitis B virus genome.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Genetic Diversity, Occult Hepatitis B, and Mutational Signatures in Migrants from Regions with Varying HBV Endemicity: Importation of Diverse Viral Variants into St. Petersburg, Russia.International journal of molecular sciences · 2026Article
- Parenteral viral hepatitis among students in Da Nang, Vietnam, and insights into new hepatitis B virus genotype B classification.Scientific reports · 2025Article
- Phylogenetic Analysis and Emerging Drug Resistance against Different Nucleoside Analogues in Hepatitis B Virus Positive Patients.Microorganisms · 2023Article
- Reconstruction of the origin and dispersal of the worldwide dominant Hepatitis B Virus subgenotype D1.Virus evolution · 2022Review
- Complex genetic encoding of the hepatitis B virus on-drug persistence.Scientific reports · 2020Article
- Complete genome analysis of hepatitis B virus in Qinghai-Tibet plateau: the geographical distribution, genetic diversity, and co-existence of HBsAg and anti-HBs antibodies.Virology journal · 2020Article
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- Transmissibility of intra-host hepatitis C virus variants.BMC genomics · 2017Article
- Accurate Genetic Detection of Hepatitis C Virus Transmissions in Outbreak Settings.The Journal of infectious diseases · 2016Article
- Advanced molecular surveillance of hepatitis C virus.Viruses · 2015Review
- Recent population expansions of hepatitis B virus in the United States.Journal of virology · 2014Article
- Hepatitis B virus transmissions associated with a portable dental clinic, West Virginia, 2009.Journal of the American Dental Association (1939) · 2013Article
- Viral quasispecies evolution.Microbiology and molecular biology reviews : MMBR · 2012Review
- Convergence and coevolution of hepatitis B virus drug resistance.Nature communications · 2012Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic analysis of hepatitis B virus (HBV) frequently involves study of intra-host variants, identification of which is commonly achieved using short regions of the HBV genome. However, the use of short sequences significantly limits evaluation of genetic relatedness among HBV strains. Although analysis of HBV complete genomes using genetic cloning has been developed, its application is highly labor intensive and practiced only infrequently. We describe here a novel approach to whole genome (WG) HBV quasispecies analysis based on end-point, limiting-dilution real-time PCR (EPLD-PCR) for amplification of single HBV genome variants, and their subsequent sequencing. EPLD-PCR was used to analyze WG quasispecies from serum samples of patients (n = 38) infected with HBV genotypes A, B, C, D, E and G. Phylogenetic analysis of the EPLD-isolated HBV-WG quasispecies showed the presence of mixed genotypes, recombinant variants and sub-populations of the virus. A critical observation was that HBV-WG consensus sequences obtained by direct sequencing of PCR fragments without EPLD are genetically close, but not always identical to the major HBV variants in the intra-host population, thus indicating that consensus sequences should be judiciously used in genetic analysis. Sequence-based studies of HBV WG quasispecies should afford a more accurate assessment of HBV evolution in various clinical and epidemiological settings.
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