ArticlePloS one2011
A functional interface at the rDNA connects rRNA synthesis, pre-rRNA processing and nucleolar surveillance in budding yeast.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Spt5's central KOW domains and the Pol II stalk collaborate to regulate chromatin and 3'-end processing in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2026Article
- New insights into nuclear import and nucleolar localization of yeast RNA exosome subunits.Molecular biology of the cell · 2025Article
- Altered rRNA processing disrupts nuclear RNA homeostasis via competition for the poly(A)-binding protein Nab2.Nucleic acids research · 2020Article
- Turnover of aberrant pre-40S pre-ribosomal particles is initiated by a novel endonucleolytic decay pathway.Nucleic acids research · 2018Article
- Noncoding RNA Surveillance: The Ends Justify the Means.Chemical reviews · 2018Review
- Feedback regulation of ribosome assembly.Current genetics · 2018Review
- Diverse Regulators of Human Ribosome Biogenesis Discovered by Changes in Nucleolar Number.Cell reports · 2018Article
- Post-Translational Dosage Compensation Buffers Genetic Perturbations to Stoichiometry of Protein Complexes.PLoS genetics · 2017Article
- Nab3 facilitates the function of the TRAMP complex in RNA processing via recruitment of Rrp6 independent of Nrd1.PLoS genetics · 2015Article
- Termination of Transcription of Short Noncoding RNAs by RNA Polymerase II.Annual review of biochemistry · 2015Review
- Structure and semi-sequence-specific RNA binding of Nrd1.Nucleic acids research · 2014Article
- Basic mechanisms in RNA polymerase I transcription of the ribosomal RNA genes.Sub-cellular biochemistry · 2013Review
- The Spt4-Spt5 complex: a multi-faceted regulator of transcription elongation.Biochimica et biophysica acta · 2013Review
- Rho and NusG suppress pervasive antisense transcription in Escherichia coli.Genes & development · 2012Article
- Transcriptome-wide analysis of exosome targets.Molecular cell · 2012Article
- Suppression analysis of esa1 mutants in Saccharomyces cerevisiae links NAB3 to transcriptional silencing and nucleolar functions.G3 (Bethesda, Md.) · 2012Article
- Trm112 is required for Bud23-mediated methylation of the 18S rRNA at position G1575.Molecular and cellular biology · 2012Article
- An overview of pre-ribosomal RNA processing in eukaryotes.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribogenesis is a multistep error-prone process that is actively monitored by quality control mechanisms. How ribosomal RNA synthesis, pre-rRNA processing and nucleolar surveillance are integrated is unclear. Nor is it understood how defective ribosomes are recognized. We report in budding yeast that, in vivo, the interaction between the transcription elongation factor Spt5 and Rpa190, the largest subunit of RNA polymerase (Pol) I, requires the Spt5 C-terminal region (CTR), a conserved and highly repetitive domain that is reminiscent of the RNA Pol II C-terminal domain (CTD). We show that this sequence is also required for the interaction between Spt5 and Nrd1, an RNA specific binding protein, and an exosome cofactor. Both the Spt4-Spt5, and the Nrd1-Nab3 complexes interact functionally with Rrp6, and colocalize at the rDNA. Mutations in the RNA binding domain of Nrd1, but not in its RNA Pol II CTD-interacting domain, and mutations in the RRM of Nab3 led to the accumulation of normal and aberrant polyadenylated pre-rRNAs. Altogether these results indicate that Nrd1-Nab3 contributes to recruiting the nucleolar surveillance to elongating polymerases to survey nascent rRNA transcripts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.