ArticleThe Journal of cell biology2011
Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon.
Article in The Journal of cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Heterogeneous translational landscape of the endoplasmic reticulum revealed by ribosome proximity labeling and transcriptome analysis.The Journal of biological chemistry · 2019Article
- Transmembrane but not soluble helices fold inside the ribosome tunnel.Nature communications · 2018Article
- Effects of protein size, thermodynamic stability, and net charge on cotranslational folding on the ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Sec61/SecY complex is inherently deficient in translocating intrinsically disordered proteins.The Journal of biological chemistry · 2017Article
- Two chaperones locked in an embrace: structure and function of the ribosome-associated complex RAC.Nature structural & molecular biology · 2017Review
- Activation of the unfolded protein response in sarcoma cells treated with rapamycin or temsirolimus.PloS one · 2017Article
- Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits.Nature structural & molecular biology · 2016Article
- Stability and flexibility of marginally hydrophobic-segment stalling at the endoplasmic reticulum translocon.Molecular biology of the cell · 2016Article
- Interaction of nascent chains with the ribosomal tunnel proteins Rpl4, Rpl17, and Rpl39 of Saccharomyces cerevisiae.The Journal of biological chemistry · 2013Article
- Functional asymmetry within the Sec61p translocon.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Structural features within the nascent chain regulate alternative targeting of secretory proteins to mitochondria.The EMBO journal · 2013Article
- The ribosome as a hub for protein quality control.Molecular cell · 2013Review
- Intra-plastid protein trafficking: how plant cells adapted prokaryotic mechanisms to the eukaryotic condition.Biochimica et biophysica acta · 2013Review
- Evolutionary conservation of codon optimality reveals hidden signatures of cotranslational folding.Nature structural & molecular biology · 2013Article
- Membrane protein TM segments are retained at the translocon during integration until the nascent chain cues FRET-detected release into bulk lipid.Molecular cell · 2012Article
- Understanding integration of α-helical membrane proteins: the next steps.Trends in biochemical sciences · 2012Article
- Arginine changes the conformation of the arginine attenuator peptide relative to the ribosome tunnel.Journal of molecular biology · 2012Article
- Tail-anchored membrane protein insertion into the endoplasmic reticulum.Nature reviews. Molecular cell biology · 2011Review
- Transmembrane segments of nascent polytopic membrane proteins control cytosol/ER targeting during membrane integration.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Multi-spanning membrane protein loops are directed alternately into the cytosol or ER lumen during cotranslational integration. Nascent chain exposure is switched after a newly synthesized transmembrane segment (TMS) enters the ribosomal tunnel. FRET measurements revealed that each TMS is initially extended, but folds into a compact conformation after moving 6-7 residues from the peptidyltransferase center, irrespective of loop size. The ribosome-induced folding of each TMS coincided with its photocrosslinking to ribosomal protein L17 and an inversion of compartmental exposure. This correlation indicates that successive TMSs fold and bind at a specific ribosomal tunnel site that includes L17, thereby triggering structural rearrangements of multiple components in and on both sides of the ER membrane, most likely via TMS-dependent L17 and/or rRNA conformational changes transmitted to the surface. Thus, cyclical changes at the membrane during integration are initiated by TMS folding, even though nascent chain conformation and location vary dynamically in the ribosome tunnel. Nascent chains therefore control their own trafficking.
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