ArticleProceedings of the National Academy of Sciences of the United States of America2011
Ultrasensitive detection of protein translocated through toxin pores in droplet-interface bilayers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Covalently Linked Peptides and Membrane Potential Enable CyaA Segment Translocation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Controlled assembly and sophisticated additive manufacturing of macroscopic droplets.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nanopore-based detection of periodontitis biomarker miR31 in saliva samples.Electrophoresis · 2024Article
- Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies.Soft matter · 2022Article
- Solution Structures ofToxins · 2021Article
- Microfluidic platform enables tailored translocation and reaction cascades in nanoliter droplet networks.Communications biology · 2020Article
- Probing membrane protein properties using droplet interface bilayers.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Chemical polyglycosylation and nanolitre detection enables single-molecule recapitulation of bacterial sugar export.Nature chemistry · 2016Article
- Studies of RNA Sequence and Structure Using Nanopores.Progress in molecular biology and translational science · 2016Review
- Nanopore sensing of botulinum toxin type B by discriminating an enzymatically cleaved Peptide from a synaptic protein synaptobrevin 2 derivative.ACS applied materials & interfaces · 2015Article
- Droplet interface bilayer reconstitution and activity measurement of the mechanosensitive channel of large conductance from Escherichia coli.Journal of the Royal Society, Interface · 2014Article
- Article
- Bordetella pertussis adenylate cyclase toxin translocation across a tethered lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Anthrax toxin-induced rupture of artificial lipid bilayer membranes.The Journal of chemical physics · 2013Article
- Constructing droplet interface bilayers from the contact of aqueous droplets in oil.Nature protocols · 2013Article
- Obstructing toxin pathways by targeted pore blockage.Chemical reviews · 2012Review
- Ratcheting up protein translocation with anthrax toxin.Protein science : a publication of the Protein Society · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Many bacterial toxins form proteinaceous pores that facilitate the translocation of soluble effector proteins across cellular membranes. With anthrax toxin this process may be monitored in real time by electrophysiology, where fluctuations in ionic current through these pores inserted in model membranes are used to infer the translocation of individual protein molecules. However, detecting the minute quantities of translocated proteins has been a challenge. Here, we describe use of the droplet-interface bilayer system to follow the movement of proteins across a model membrane separating two submicroliter aqueous droplets. We report the capture and subsequent direct detection of as few as 100 protein molecules that have translocated through anthrax toxin pores. The droplet-interface bilayer system offers new avenues of approach to the study of protein translocation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.