ArticlePlant physiology2011
Toward the storage metabolome: profiling the barley vacuole.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 99 citations in OpenAlex.
- PERSPECTIVE: organelle positioning as a principle of metabolic regulation and stress tolerance.The Plant journal : for cell and molecular biology · 2026Review
- Photosynthesis, Anatomy, and Metabolism as a Tool for Assessing Physiological Modulation in Five Native Species of the Brazilian Atlantic Forest.Plants (Basel, Switzerland) · 2024Article
- Plant Heterotrophic Cultures: No Food, No Growth.Plants (Basel, Switzerland) · 2024Review
- Past accomplishments and future challenges of the multi-omics characterization of leaf growth.Plant physiology · 2022Review
- The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole.Metabolites · 2021Article
- Gluconeogenesis in Plants: A Key Interface between Organic Acid/Amino Acid/Lipid and Sugar Metabolism.Molecules (Basel, Switzerland) · 2021Review
- Sucrose Metabolism and Transport in Grapevines, with Emphasis on Berries and Leaves, and Insights Gained from a Cross-Species Comparison.International journal of molecular sciences · 2021Review
- Phenolic Metabolites from Barley in Contribution to Phenome in soil Moisture Deficit.International journal of molecular sciences · 2020Article
- Salt stress induced differential metabolic responses in the sprouting tubers of Jerusalem artichoke (Helianthus tuberosus L.).PloS one · 2020Article
- Niche-specific metabolic adaptation in biotrophic and necrotrophic oomycetes is manifested in differential use of nutrients, variation in gene content, and enzyme evolution.PLoS pathogens · 2019Article
- Induced, Imprinted, and Primed Responses to Changing Environments: Does Metabolism Store and Process Information?Frontiers in plant science · 2019Article
- Vacuolar Transporters - Companions on a Longtime Journey.Plant physiology · 2018Review
- Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense.Journal of experimental botany · 2017Article
- Network-Guided GWAS Improves Identification of Genes Affecting Free Amino Acids.Plant physiology · 2017Article
- The Interplay between Carbon Availability and Growth in Different Zones of the Growing Maize Leaf.Plant physiology · 2016Article
- Article
- Integrative Approaches to Enhance Understanding of Plant Metabolic Pathway Structure and Regulation.Plant physiology · 2015Article
- Identification of a plastidial phenylalanine exporter that influences flux distribution through the phenylalanine biosynthetic network.Nature communications · 2015Article
- Mitochondrial Dihydrolipoyl Dehydrogenase Activity Shapes Photosynthesis and Photorespiration of Arabidopsis thaliana.The Plant cell · 2015Article
- Molecular Composition of Plant Vacuoles: Important but Less Understood Regulations and Roles of Tonoplast Lipids.Plants (Basel, Switzerland) · 2015Review
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While recent years have witnessed dramatic advances in our capacity to identify and quantify an ever-increasing number of plant metabolites, our understanding of how metabolism is spatially regulated is still far from complete. In an attempt to partially address this question, we studied the storage metabolome of the barley (Hordeum vulgare) vacuole. For this purpose, we used highly purified vacuoles isolated by silicon oil centrifugation and compared their metabolome with that found in the mesophyll protoplast from which they were derived. Using a combination of gas chromatography-mass spectrometry and Fourier transform-mass spectrometry, we were able to detect 59 (primary) metabolites for which we know the exact chemical structure and a further 200 (secondary) metabolites for which we have strong predicted chemical formulae. Taken together, these metabolites comprise amino acids, organic acids, sugars, sugar alcohols, shikimate pathway intermediates, vitamins, phenylpropanoids, and flavonoids. Of the 259 putative metabolites, some 12 were found exclusively in the vacuole and 34 were found exclusively in the protoplast, while 213 were common in both samples. When analyzed on a quantitative basis, however, there is even more variance, with more than 60 of these compounds being present above the detection limit of our protocols. The combined data were also analyzed with respect to the tonoplast proteome in an attempt to infer specificities of the transporter proteins embedded in this membrane. Following comparison with recent observations made using nonaqueous fractionation of Arabidopsis (Arabidopsis thaliana), we discuss these data in the context of current models of metabolic compartmentation in plants.
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