ArticleJournal of bacteriology2011
Biochemical and cellular characterization of Helicobacter pylori RecA, a protein with high-level constitutive expression.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- A new component of the DNA damage response biofilm axis in Acinetobacter baumannii is a TetR-like DNA damage response regulator.FEMS microbiology letters · 2026Article
- Functional Division Between the RecA1 and RecA2 Proteins inFrontiers in microbiology · 2020Article
- Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori.Nature communications · 2019Article
- Acid-responsive activity of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- N4-cytosine DNA methylation regulates transcription and pathogenesis in Helicobacter pylori.Nucleic acids research · 2018Article
- Helicobacter pylori gene silencing in vivo demonstrates urease is essential for chronic infection.PLoS pathogens · 2017Article
- Homeostasis of metabolites inRoyal Society open science · 2016Article
- Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach.Scientific reports · 2016Article
- Mutations in the nucleotide binding and hydrolysis domains of Helicobacter pylori MutS2 lead to altered biochemical activities and inactivation of its in vivo function.BMC microbiology · 2016Article
- Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005.MicrobiologyOpen · 2015Article
- The BER necessities: the repair of DNA damage in human-adapted bacterial pathogens.Nature reviews. Microbiology · 2015Review
- Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays.PloS one · 2015Article
- Life in the human stomach: persistence strategies of the bacterial pathogen Helicobacter pylori.Nature reviews. Microbiology · 2013Review
- The cell pole: the site of cross talk between the DNA uptake and genetic recombination machinery.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter pylori is a bacterial pathogen colonizing half of the world's human population. It has been implicated in a number of gastric diseases, from asymptomatic gastritis to cancer. It is characterized by an amazing genetic variability that results from high mutation rates and efficient DNA homologous recombination and transformation systems. Here, we report the characterization of H. pylori RecA (HpRecA), a protein shown to be involved in DNA repair, transformation, and mouse colonization. The biochemical characterization of the purified recombinase reveals activities similar to those of Escherichia coli RecA (EcRecA). We show that in H. pylori, HpRecA is present in about 80,000 copies per cell during exponential growth and decreases to about 50,000 copies in stationary phase. The amount of HpRecA remains unchanged after induction of DNA lesions, suggesting that HpRecA is always expressed at a high level in order to repair DNA damage or facilitate recombination. We performed HpRecA localization analysis by adding a Flag tag to the protein, revealing two different patterns of localization. During exponential growth, RecA-Flag presents a diffuse pattern, overlapping with the DAPI (4',6-diamidino-2-phenylindole) staining of DNA, whereas during stationary phase, the protein is present in more defined areas devoid of DAPI staining. These localizations are not affected by inactivation of competence or DNA recombination genes. Neither UV irradiation nor gamma irradiation modified HpRecA localization, suggesting the existence of a constitutive DNA damage adaptation system.
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