ArticleJournal of lipid research2011
Identification of cholesterol crystals in plaques of atherosclerotic mice using hyperspectral CARS imaging.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 110 citations in OpenAlex.
- Systematic Overview of Solid Particles and Their Host Responses.Frontiers in immunology · 2018Pooled it
- Differentiation of sphingomyelin and cholesterol by hyperspectral mid-infrared detection of single-bond vibrational modes in the fingerprint region.Nature methods · 2026Article
- Advancing Label-Free Imaging Through CARS Microscopy: From Signal Formation to Biological Interpretation.International journal of molecular sciences · 2026Review
- Identification of Cholesterol in Plaques of Atherosclerotic Using Magnetic Resonance Spectroscopy and 1D U-Net Architecture.Molecules (Basel, Switzerland) · 2026Article
- Preventing Age-Related Nuclear Cataract Development - Is Cholesterol the Key?Investigative ophthalmology & visual science · 2026Review
- Vascular smooth muscle cells in response to cholesterol crystals modulates inflammatory cytokines release and promotes neutrophil extracellular trap formation.Molecular medicine (Cambridge, Mass.) · 2024Article
- Bibliometric evaluation of publications on inflammasomes in atherosclerosis from 2002 to 2022.Frontiers in cardiovascular medicine · 2023Article
- Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer's Disease Mouse Model with Combined TPEF/CARS Microscopy.Biomedicines · 2022Article
- Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.Photoacoustics · 2022Article
- Liver-humanized mice: A translational strategy to study metabolic disorders.Journal of cellular physiology · 2022Review
- Formation and Cellular Impact of Cholesterol Crystals in Health and Disease.Advanced biology · 2021Review
- Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoEScientific reports · 2021Article
- Article
- Bile acid sequestration reverses liver injury and prevents progression of nonalcoholic steatohepatitis in Western diet-fed mice.The Journal of biological chemistry · 2020Article
- Coherent anti-Stokes Raman spectroscopy of single and multi-layer graphene.Nature communications · 2019Article
- Ultramorphological analysis of plaque advancement and cholesterol crystal formation in Ldlr knockout mouse atherosclerosis.Atherosclerosis · 2019Article
- Why Is Very High Cholesterol Content Beneficial for the Eye Lens but Negative for Other Organs?Nutrients · 2019Review
- The physiology of foamy phagocytes in multiple sclerosis.Acta neuropathologica communications · 2018Review
- Hyperspectral imaging solutions for brain tissue metabolic and hemodynamic monitoring: past, current and future developments.Journal of optics (2010) · 2018Review
- Cholesterol Crystal-Mediated Inflammation Is Driven by Plasma Membrane Destabilization.Frontiers in immunology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The accumulation of lipids, including cholesterol, in the arterial wall plays a key role in the pathogenesis of atherosclerosis. Although several advances have been made in the detection and imaging of these lipid structures in plaque lesions, their morphology and composition have yet to be fully elucidated, particularly in different animal models of disease. To address this issue, we analyzed lipid morphology and composition in the atherosclerotic plaques of two animal models of disease, the low density lipoprotein receptor-deficient (LDLR(-/-)) mouse and the ApoE lipoprotein-deficient (ApoE(-/-)) mouse, utilizing hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy in combination with principal component analysis (PCA). Hyperspectral CARS imaging revealed lipid-rich macrophage cells and condensed needle-shaped and plate-shaped lipid crystal structures in both mice. Spectral analysis with PCA and comparison to spectra of pure cholesterol and cholesteryl ester derivatives further revealed these lipid structures to be pure cholesterol crystals, which were predominantly observed in the ApoE(-/-) mouse model. These results illustrate the ability of hyperspectral CARS imaging in combination with multivariate analysis to characterize atherosclerotic lipid morphology and composition with chemical specificity, and consequently, provide new insight into the formation of cholesterol crystal structures in atherosclerotic plaque lesions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.