ArticleApplied and environmental microbiology2011
Proteomic and functional analysis of the cellulase system expressed by Postia placenta during brown rot of solid wood.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Basidiomycota Fungi and ROS: Genomic Perspective on Key Enzymes Involved in Generation and Mitigation of Reactive Oxygen Species.Frontiers in fungal biology · 2022Review
- Retracted and Republished from: "Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot FungusApplied and environmental microbiology · 2021Article
- Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation.Frontiers in fungal biology · 2021Article
- Transcriptome analysis of the brown rot fungus Gloeophyllum trabeum during lignocellulose degradation.PloS one · 2020Article
- Degradative Capacity of Two Strains ofFrontiers in microbiology · 2020Article
- The Foliar EndophyteMicrobiology resource announcements · 2019Article
- Multi-omic Analyses of Extensively Decayed Pinus contorta Reveal Expression of a Diverse Array of Lignocellulose-Degrading Enzymes.Applied and environmental microbiology · 2018Article
- Insight into metabolic diversity of the brown-rot basidiomycete Postia placenta responsible for sesquiterpene biosynthesis: semi-comprehensive screening of cytochrome P450 monooxygenase involved in protoilludene metabolism.Microbial biotechnology · 2018Article
- Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola.Applied and environmental microbiology · 2018Article
- Article
- Draft genome sequence of a monokaryotic model brown-rot fungusGenomics data · 2017Article
- Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Transcriptome and Secretome Analyses of the Wood Decay Fungus Wolfiporia cocos Support Alternative Mechanisms of Lignocellulose Conversion.Applied and environmental microbiology · 2016Article
- A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus.Biotechnology for biofuels · 2016Article
- Destructuring plant biomass: focus on fungal and extremophilic cell wall hydrolases.Plant science : an international journal of experimental plant biology · 2015Review
- Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood.PLoS genetics · 2014Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brown rot basidiomycetes have an important ecological role in lignocellulose recycling and are notable for their rapid degradation of wood polymers via oxidative and hydrolytic mechanisms. However, most of these fungi apparently lack processive (exo-acting) cellulases, such as cellobiohydrolases, which are generally required for efficient cellulolysis. The recent sequencing of the Postia placenta genome now permits a proteomic approach to this longstanding conundrum. We grew P. placenta on solid aspen wood, extracted proteins from the biodegrading substrate, and analyzed tryptic digests by shotgun liquid chromatography-tandem mass spectrometry. Comparison of the data with the predicted P. placenta proteome revealed the presence of 34 likely glycoside hydrolases, but only four of these--two in glycoside hydrolase family 5, one in family 10, and one in family 12--have sequences that suggested possible activity on cellulose. We expressed these enzymes heterologously and determined that they all exhibited endoglucanase activity on phosphoric acid-swollen cellulose. They also slowly hydrolyzed filter paper, a more crystalline substrate, but the soluble/insoluble reducing sugar ratios they produced classify them as nonprocessive. Computer simulations indicated that these enzymes produced soluble/insoluble ratios on reduced phosphoric acid-swollen cellulose that were higher than expected for random hydrolysis, which suggests that they could possess limited exo activity, but they are at best 10-fold less processive than cellobiohydrolases. It appears likely that P. placenta employs a combination of oxidative mechanisms and endo-acting cellulases to degrade cellulose efficiently in the absence of a significant processive component.
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