ArticleApplied and environmental microbiology2011
Hemocytes are sites of enteric virus persistence within oysters.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Research Progress on Biological Accumulation, Detection and Inactivation Technologies of Norovirus in Oysters.Foods (Basel, Switzerland) · 2023Review
- Bioaccumulation Dynamic by Crassostrea gigas Oysters of Viruses That Are Proposed as Surrogates for Enteric Virus Contamination in Environmental Samples.Food and environmental virology · 2023Article
- Assessment of the Impact on Human Health of the Presence of Norovirus in Bivalve Molluscs: What Data Do We Miss?Foods (Basel, Switzerland) · 2021Review
- Quantitative Real-Time PCR and Digital PCR to Evaluate Residual Quantity of HAV in Experimentally Depurated Mussels.Food and environmental virology · 2021Article
- Norovirus Persistence in Oysters to Prolonged Commercial Purification.Pathogens (Basel, Switzerland) · 2021Article
- Article
- Persistence of Hepatitis A Virus RNA in Water, on Non-porous Surfaces, and on Blueberries.Frontiers in microbiology · 2021Article
- F-Specific RNA Bacteriophages Model the Behavior of Human Noroviruses during Purification of Oysters: the Main Mechanism Is Probably Inactivation Rather than Release.Applied and environmental microbiology · 2020Article
- From Species to Regional and Local Specialization of Intestinal Macrophages.Frontiers in cell and developmental biology · 2020Review
- Synergistic Effects of Combined Chlorine and Vitamin BFood and environmental virology · 2019Article
- Evaluation of a Male-Specific DNA Coliphage Persistence Within Eastern Oysters (Crassostrea virginica).Food and environmental virology · 2019Article
- Current and Emerging Technologies for the Detection of Norovirus from Shellfish.Foods (Basel, Switzerland) · 2019Article
- High turnover of faecal microbiome from algal feedstock experimental manipulations in the Pacific oyster (Crassostrea gigas).Microbial biotechnology · 2018Article
- Persistence of MS-2 Bacteriophage Within Eastern Oysters.Food and environmental virology · 2018Article
- Selected Viruses Detected on and in our Food.Current clinical microbiology reports · 2018Review
- Occurrence and Trend of Hepatitis A Virus in Bivalve Molluscs Production Areas Following a Contamination Event.Food and environmental virology · 2017Article
- Temperature-Dependent Persistence of Human Norovirus Within Oysters (Crassostrea virginica).Food and environmental virology · 2016Article
- Thermal Inactivation of Enteric Viruses and Bioaccumulation of Enteric Foodborne Viruses in Live Oysters (Crassostrea virginica).Applied and environmental microbiology · 2016Article
- A Gnotobiotic Pig Model for Determining Human Norovirus Inactivation by High-Pressure Processing.Applied and environmental microbiology · 2015Article
- Effect of temperature and relative humidity on the survival of foodborne viruses during food storage.Applied and environmental microbiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The goal of this study was to determine how enteric viruses persist within shellfish tissues. Several lines of novel evidence show that phagocytic blood cells (hemocytes) of Eastern oysters (Crassostrea virginica) play an important role in the retention of virus particles. Our results demonstrated an association of virus contamination with hemocytes but not with hemolymph. Live oysters contaminated overnight with hepatitis A virus (HAV) and murine norovirus (MNV) had 56% and 80% of extractable virus associated with hemocytes, respectively. Transfer of HAV-contaminated hemocytes to naïve (virus-free) oysters resulted in naïve oyster meat testing HAV positive for up to 3 weeks. Acid tolerance of HAV, MNV, poliovirus (PV), and feline calicivirus (FCV) correlated with the ability of each virus to persist within oysters. Using reverse transcription-PCR (RT-PCR) to evaluate persistence of these viruses in oysters, we showed that HAV persisted the longest (>21 days) and was most acid resistant, MNV and PV were less tolerant of acidic pH, persisting for up to 12 days and 1 day, respectively, and FCV did not persist (<1 day) within oysters and was not acid tolerant. This suggests that the ability of a virus to tolerate the acidic conditions typical of phagolysosomal vesicles within hemocytes plays a role in determining virus persistence in shellfish. Evaluating oyster and hemocyte homogenates and live contaminated oysters as a prelude to developing improved viral RNA extraction methods, we found that viruses were extracted more expediently from hemocytes than from whole shellfish tissues and gave similar RT-PCR detection sensitivities.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.