ArticleApplied and environmental microbiology2011
Activity-based metagenomic screening and biochemical characterization of bovine ruminal protozoan glycoside hydrolases.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 57 citations in OpenAlex.
- Temporal dynamics shifts in multi-kingdom rumen microbiota and fermentation profiles of water buffalo under heat stress.Animal microbiome · 2026Article
- - Invited Review - Ruminal ciliates as modulators of the rumen microbiome.Animal bioscience · 2024Article
- Genomic insights into the phylogeny and biomass-degrading enzymes of rumen ciliates.The ISME journal · 2022Article
- Review
- A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.The Journal of biological chemistry · 2020Article
- Rumen Protozoa Play a Significant Role in Fungal Predation and Plant Carbohydrate Breakdown.Frontiers in microbiology · 2020Article
- Islands in the stream: from individual to communal fiber degradation in the rumen ecosystem.FEMS microbiology reviews · 2019Review
- Recent Advances in Function-based Metagenomic Screening.Genomics, proteomics & bioinformatics · 2018Review
- Directly mining a fungal thermostable α-amylase from Chinese Nong-flavor liquor starter.Microbial cell factories · 2018Article
- Repeated inoculation of cattle rumen with bison rumen contents alters the rumen microbiome and improves nitrogen digestibility in cattle.Scientific reports · 2017Article
- New roles in hemicellulosic sugar fermentation for the uncultivated Bacteroidetes family BS11.The ISME journal · 2017Article
- New Primers Targeting Full-Length Ciliate 18S rRNA Genes and Evaluation of Dietary Effect on Rumen Ciliate Diversity in Dairy Cows.Current microbiology · 2015Article
- Metatranscriptomic analyses of plant cell wall polysaccharide degradation by microorganisms in the cow rumen.Applied and environmental microbiology · 2015Article
- The Role of Ciliate Protozoa in the Rumen.Frontiers in microbiology · 2015Review
- Discovery of new protein families and functions: new challenges in functional metagenomics for biotechnologies and microbial ecology.Frontiers in microbiology · 2015Review
- Metatranscriptomics reveals the diversity of genes expressed by eukaryotes in forest soils.PloS one · 2012Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rumen, the foregut of herbivorous ruminant animals such as cattle, functions as a bioreactor to process complex plant material. Among the numerous and diverse microbes involved in ruminal digestion are the ruminal protozoans, which are single-celled, ciliated eukaryotic organisms. An activity-based screen was executed to identify genes encoding fibrolytic enzymes present in the metatranscriptome of a bovine ruminal protozoan-enriched cDNA expression library. Of the four novel genes identified, two were characterized in biochemical assays. Our results provide evidence for the effective use of functional metagenomics to retrieve novel enzymes from microbial populations that cannot be maintained in axenic cultures.
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