ArticleApplied and environmental microbiology2011
Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Advances in the biological production of butyric acid.World journal of microbiology & biotechnology · 2026Review
- Iron plaques as terminal electron acceptors optimize clostridial fermentation and nitrogen fixation in rice rhizospheres.The ISME journal · 2026Article
- Comparative Metabolomics ofJournal of microbiology and biotechnology · 2025Article
- Metabolomics: Going Deeper, Going Broader, Going Further.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Crystal Structure and Molecular Mechanism of Phosphotransbutyrylase fromJournal of microbiology and biotechnology · 2021Article
- Renewable fatty acid ester production in Clostridium.Nature communications · 2021Article
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- Genetic sensor-regulators functional in Clostridia.Journal of industrial microbiology & biotechnology · 2020Article
- The Iron-Sulfur Flavoprotein DsrL as NAD(P)H:Acceptor Oxidoreductase in Oxidative and Reductive Dissimilatory Sulfur Metabolism.Frontiers in microbiology · 2020Article
- Impact of iron reduction on the metabolism of Clostridium acetobutylicum.Environmental microbiology · 2019Article
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- Microbial Organic Matter Degradation Potential in Baltic Sea Sediments Is Influenced by Depositional Conditions andApplied and environmental microbiology · 2019Article
- Discovery and implementation of a novel pathway forBiotechnology for biofuels · 2019Article
- Omics-based analyses revealed metabolic responses ofBiotechnology for biofuels · 2019Article
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- Metabolism the Difficile Way: The Key to the Success of the PathogenFrontiers in microbiology · 2019Review
- Reviving the Weizmann process for commercial n-butanol production.Nature communications · 2018Article
- Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate ofSaudi journal of biological sciences · 2018Article
- HBiotechnology for biofuels · 2018Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fermentation carried out by the biofuel producer Clostridium acetobutylicum is characterized by two distinct phases. Acidogenesis occurs during exponential growth and involves the rapid production of acids (acetate and butyrate). Solventogenesis initiates as cell growth slows down and involves the production of solvents (butanol, acetone, and ethanol). Using metabolomics, isotope tracers, and quantitative flux modeling, we have mapped the metabolic changes associated with the acidogenic-solventogenic transition. We observed a remarkably ordered series of metabolite concentration changes, involving almost all of the 114 measured metabolites, as the fermentation progresses from acidogenesis to solventogenesis. The intracellular levels of highly abundant amino acids and upper glycolytic intermediates decrease sharply during this transition. NAD(P)H and nucleotide triphosphates levels also decrease during solventogenesis, while low-energy nucleotides accumulate. These changes in metabolite concentrations are accompanied by large changes in intracellular metabolic fluxes. During solventogenesis, carbon flux into amino acids, as well as flux from pyruvate (the last metabolite in glycolysis) into oxaloacetate, decreases by more than 10-fold. This redirects carbon into acetyl coenzyme A, which cascades into solventogenesis. In addition, the electron-consuming reductive tricarboxylic acid (TCA) cycle is shutdown, while the electron-producing oxidative (clockwise) right side of the TCA cycle remains active. Thus, the solventogenic transition involves global remodeling of metabolism to redirect resources (carbon and reducing power) from biomass production into solvent production.
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