ArticleStem cells (Dayton, Ohio)2011
Phosphatase and tensin homolog regulates the pluripotent state and lineage fate choice in human embryonic stem cells.
Article in Stem cells (Dayton, Ohio), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Chicken Primordial Germ Cells Do Not Proliferate in Insulin-Lacking Media.International journal of molecular sciences · 2025Article
- Therapeutic role of PTEN in tissue regeneration for management of neurological disorders: stem cell behaviors to an in-depth review.Cell death & disease · 2024Review
- Pleiotropic roles of tankyrase/PARP proteins in the establishment and maintenance of human naïve pluripotency.Experimental cell research · 2020Review
- Derivation of induced pluripotent stem cells from ferret somatic cells.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- PI3K in stemness regulation: from development to cancer.Biochemical Society transactions · 2020Review
- Wenyang Jieyu Decoction Alleviates Depressive Behavior in the Rat Model of Depression via Regulation of the Intestinal Microbiota.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- PTEN: A Thrifty Gene That Causes Disease in Times of Plenty?Frontiers in nutrition · 2020Article
- OncogenicProceedings of the National Academy of Sciences of the United States of America · 2019Article
- PTEN Inhibition in Human Disease Therapy.Molecules (Basel, Switzerland) · 2018Review
- HIF-2α and Oct4 have synergistic effects on survival and myocardial repair of very small embryonic-like mesenchymal stem cells in infarcted hearts.Cell death & disease · 2017Article
- Utility of Lymphoblastoid Cell Lines for Induced Pluripotent Stem Cell Generation.Stem cells international · 2016Article
- REST/NRSF Knockdown Alters Survival, Lineage Differentiation and Signaling in Human Embryonic Stem Cells.PloS one · 2015Article
- Quantitative Analysis of Robustness of Dynamic Response and Signal Transfer in Insulin mediated PI3K/AKT Pathway.Computers & chemical engineering · 2014Article
- Signals that regulate the oncogenic fate of neural stem cells and progenitors.Experimental neurology · 2014Review
- Testicular cancer: biology and biomarkers.Virchows Archiv : an international journal of pathology · 2014Review
- Protein post-translational modifications and regulation of pluripotency in human stem cells.Cell research · 2014Review
- Protein kinases and associated pathways in pluripotent state and lineage differentiation.Current stem cell research & therapy · 2014Review
- Inhibition of PTEN tumor suppressor promotes the generation of induced pluripotent stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Understanding the intrinsic and extrinsic signals that regulate the molecular basis of the pluripotent state may improve our understanding of mammalian embryogenesis, different states of pluripotency, and our ability to tailor lineage differentiation. Although the role of the PI3K/Akt pathway in the self-renewal and maintenance of mESCs is well-established, the specific contribution of the pathway or of its negative regulator, PTEN, in the maintenance of the human pluripotent state is less understood. To explore the PI3K/AKT pathway in human embryonic stem cell (hESC) pluripotency and differentiation, we generated stable PTEN knockdown (KD) hESCs using short hairpin RNA. Similar to mESCs, we found that PTEN KD hESCs have increased self-renewal, cell survival, and proliferation over multiple passages compared to control cells. However, in contrast to mESCs, in vitro, PTEN KD hESCs differentiated inefficiently in directed differentiation assays, in part due to the continued maintenance of OCT4 and NANOG expression. In teratoma assays, PTEN KD hESCs generated tissues from the three germ layers, although with a bias toward neuroectoderm differentiation. These results demonstrate that PTEN is a key regulator of hESC growth and differentiation, and manipulation of this pathway may improve our ability to regulate and understand the pluripotent state.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.