Evidence map›Paper›PMID 21948286›Full record

ArticleFolia microbiologica2011

Detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification.

Chun Wang, Victor Fei Pang, Chian-Ren Jeng, Fan Lee, Yu-Wen Huang, Yeou-Liang Lin, Shih-Hsuan Hsiao, Shiow-Suey Lai

Open access · hybridAbstract readEvaluation Study
In one paragraph

Article in Folia microbiologica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Reservoirs of Porcine Circoviruses: A Mini Review.Frontiers in veterinary science · 2019
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Chun WangAnimal Health Research Institute, Council of Agriculture, Executive Yuan, No. 376, Chung-Cheng Road, Tansui, New Taipei City 25158, Taiwan.
Victor Fei Pang
Chian-Ren Jeng
Fan Lee
Yu-Wen Huang
Yeou-Liang Lin
Shih-Hsuan Hsiao
Shiow-Suey Lai
National Taiwan University · TWAnimal Health Research Institute · TWCouncil of Agriculture · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A loop-mediated isothermal amplification (LAMP) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (PCV1) in commercial swine vaccines. This method was highly specific for PCV1. No cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. The analytical sensitivity of the LAMP for PCV1 DNA was 10 copies/μl in the case of positive recombinant plasmid comparable to that obtained from the nested polymerase chain reaction (nested PCR). Furthermore, 25 commercial swine vaccines were tested by both the LAMP and the nested PCR, and three of them were tested positive for PCV1 DNA. These results indicate that PCV1 DNA can be real-time detected by the LAMP; the method was highly specific, sensitive, and rapid for the detection of PCV1 DNA, particularly in commercial swine vaccines.

Indexed as

AnimalsCircoviridae InfectionsCircovirusDNA PrimersNucleic Acid Amplification TechniquesSwineSwine DiseasesViral VaccinesDNA PrimersViral Vaccines

Identifiers

PMID21948286
PMCPMC3251781
OpenAlexW2091276882

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.