ArticleClinical cancer research : an official journal of the American Association for Cancer Research2011
Molecular imaging of TGFβ-induced Smad2/3 phosphorylation reveals a role for receptor tyrosine kinases in modulating TGFβ signaling.
Article in Clinical cancer research : an official journal of the American Association for Cancer Research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
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- miR-218-5p Induces Interleukin-1β and Endovascular Trophoblast Differentiation by Targeting the Transforming Growth Factor β-SMAD2 Pathway.Frontiers in endocrinology · 2022Article
- TGFBR2 mediated phosphorylation of BUB1 at Ser-318 is required for transforming growth factor-β signaling.Neoplasia (New York, N.Y.) · 2020Article
- Short-term cyclical stretch phosphorylates p38 and ERK1/2 MAPKs in cultured fibroblasts from the hearts of rainbow trout,Biology open · 2020Article
- Upregulation of Extracellular Vesicles-Encapsulated miR-132 Released From Mesenchymal Stem Cells Attenuates Ischemic Neuronal Injury by Inhibiting Smad2/c-jun PathwayFrontiers in cell and developmental biology · 2020Article
- Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Review
- Adipose-derived stromal cell secretome disrupts autophagy in glioblastoma.Journal of molecular medicine (Berlin, Germany) · 2019Article
- A novel reporter for real-time, quantitative imaging of AKT-directed K63-poly-ubiquitination in living cells.Oncotarget · 2018Article
- Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase.Methods in molecular biology (Clifton, N.J.) · 2018Article
- TRPM7 kinase activity is essential for T cell colonization and alloreactivity in the gut.Nature communications · 2017Article
- Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Quantitative and Dynamic Imaging of ATM Kinase Activity.Methods in molecular biology (Clifton, N.J.) · 2017Article
- A Requirement for ZAK Kinase Activity in Canonical TGF-β Signaling.Translational oncology · 2016Article
- Novel Bioluminescent Activatable Reporter for Src Tyrosine Kinase Activity in Living Mice.Theranostics · 2016Article
- The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-β signaling.Science signaling · 2015Article
- Curcumin and emodin down-regulate TGF-β signaling pathway in human cervical cancer cells.PloS one · 2015Article
- Reproducible combinatorial regulatory networks elucidate novel oncogenic microRNAs in non-small cell lung cancer.RNA (New York, N.Y.) · 2014Article
- Laminin α1 regulates age-related mesangial cell proliferation and mesangial matrix accumulation through the TGF-β pathway.The American journal of pathology · 2014Article
Corrections and comments
- Erratum issuedNyati, Mukesh [corrected to Nyati, Mukesh K]
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe dual modality of TGFβ, both as a potent tumor suppressor and a stimulator of tumor progression, invasion, and metastasis, make it a critical target for therapeutic intervention in human cancers. The ability to carry out real-time, noninvasive imaging of TGFβ-activated Smad signaling in live cells and animal models would significantly improve our understanding of the regulation of this unique signaling cascade. To advance these efforts, we developed a highly sensitive molecular imaging tool that repetitively, noninvasively, and dynamically reports on TGFBR1 kinase activity. EXPERIMENTAL
designThe bioluminescent TGFβR1 reporter construct was developed using a split firefly luciferase gene containing a functional sensor of Smad2 phosphorylation, wherein inhibition of TGFβ receptor1 kinase activity leads to an increase in reporter signaling. The reporter was stably transfected into mammalian cells and used to image in vivo and in vitro bioluminescent activity as a surrogate for monitoring TGFBR1 kinase activity.
resultsThe reporter was successfully used to monitor direct and indirect inhibition of TGFβ-induced Smad2 and SMAD3 phosphorylation in live cells and tumor xenografts and adapted for high-throughput screening, to identify a role for receptor tyrosine kinase inhibitors as modulators of TGFβ signaling.
conclusionThe reporter is a dynamic, noninvasive imaging modality for monitoring TGFβ-induced Smad2 signaling in live cells and tumor xenografts. It has immense potential for identifying novel effectors of R-Smad phosphorylation, for validating drug-target interaction, and for studying TGFβ signaling in different metastasis models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.