Evidence map›Paper›PMID 21947784›Full record

ArticleTransgenic research2012

Utilization of PVX-Cre expression vector in potato.

Lilya Kopertekh, Veronica v Saint Paul, Erika Krebs, Joachim Schiemann

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Lilya KopertekhJulius Kuehn Institute, Federal Research Centre for Cultivated Plants (JKI), Institute for Biosafety of Genetically Modified Plants, Erwin-Baur-Str 27, 06484 Quedlinburg, Germany.
Veronica v Saint Paul
Erika Krebs
Joachim Schiemann
Julius Kühn-Institut · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trait genes are usually introduced into the plant genome together with a marker gene. The last one becomes unnecessary after transgene selection and characterization. One of the strategies to produce transgenic plants free from the selectable marker is based on site-specific recombination. The present study employed the transient Cre-lox system to remove the nptII marker gene from potato. Transient marker gene excision involves introduction of Cre protein in lox-target plants by PVX virus vector followed by plant regeneration. Using optimized experimental conditions, such as particle bombardment infection method and application of P19 silencing suppressor protein, 20-27% of regenerated plants were identified by PCR analysis as marker-free. Based on our comparison of the recombination frequencies observed in this study to the efficiency of other methods to avoid or eliminate marker genes in potato, we suggest that PVX-Cre mediated site-specific excisional recombination is a useful tool to generate potato plants without superfluous transgenic sequences.

Indexed as

Agrobacterium tumefaciensBlotting, SouthernCapsid ProteinsDNA, PlantGene SilencingGenes, ReporterGenes, SuppressorGenetic VectorsGreen Fluorescent ProteinsIntegrasesPlant LeavesPlants, Genetically ModifiedPlasmidsPotexvirusRecombination, GeneticSolanum tuberosumCapsid ProteinsCre recombinaseDNA, PlantGreen Fluorescent ProteinsIntegrasesP19 protein, tomato bushy stunt virusViral Proteins

Identifiers

PMID21947784
OpenAlexW2025781842

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.