ReviewMetabolic engineering2012
Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways.
Review in Metabolic engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 146 citations in OpenAlex.
- Organ-on-a-Chip and Microfluidic Plant Cell Culture Systems: The Next Frontier for Controlled Secondary Metabolite Production and Real-Time Metabolomic Monitoring.Plants (Basel, Switzerland) · 2026Review
- Advancing lignocellulosic conversion though biosensor-enabled metabolic engineering.Green chemistry : an international journal and green chemistry resource : GC · 2025Review
- Programming Nutrient Detection with Modular Regulators for Dynamic Control of Microbial Biosynthesis.ACS synthetic biology · 2025Article
- Genetic Circuits for Feedback Control of Gamma-Aminobutyric Acid Biosynthesis in ProbioticMetabolites · 2024Article
- AraC-Based Biosensor for the Detection of Isoprene inACS omega · 2023Article
- The Establishment of a Tobramycin-Responsive Whole-Cell Micro-Biosensor Based on an Artificial Ribozyme Switch.Life (Basel, Switzerland) · 2023Article
- Real-Time Assessment of Intracellular Metabolites in Single Cells through RNA-Based Sensors.Biomolecules · 2023Review
- Biosensor-enabled pathway optimization in metabolic engineering.Current opinion in biotechnology · 2022Review
- Cell-based high-throughput screening of polysaccharide biosynthesis hosts.Microbial cell factories · 2021Review
- A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors.Nature communications · 2021Article
- Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae.Microbial cell factories · 2020Article
- Branch point control at malonyl-CoA node: A computational framework to uncover the design principles of an ideal genetic-metabolic switch.Metabolic engineering communications · 2020Article
- Article
- Tools and systems for evolutionary engineering of biomolecules and microorganisms.Journal of industrial microbiology & biotechnology · 2019Review
- Bacterial Metabolism and Antibiotic Efficacy.Cell metabolism · 2019Review
- Engineering metabolite-responsive transcriptional factors to sense small molecules in eukaryotes: current state and perspectives.Microbial cell factories · 2019Review
- Genetically encoded biosensors for lignocellulose valorization.Biotechnology for biofuels · 2019Review
- Dynamic control of endogenous metabolism with combinatorial logic circuits.Molecular systems biology · 2018Article
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cells are filled with biosensors, molecular systems that measure the state of the cell and respond by regulating host processes. In much the same way that an engineer would monitor a chemical reactor, the cell uses these sensors to monitor changing intracellular environments and produce consistent behavior despite the variable environment. While natural systems derive a clear benefit from pathway regulation, past research efforts in engineering cellular metabolism have focused on introducing new pathways and removing existing pathway regulation. Synthetic biology is a rapidly growing field that focuses on the development of new tools that support the design, construction, and optimization of biological systems. Recent advances have been made in the design of genetically-encoded biosensors and the application of this class of molecular tools for optimizing and regulating heterologous pathways. Biosensors to cellular metabolites can be taken directly from natural systems, engineered from natural sensors, or constructed entirely in vitro. When linked to reporters, such as antibiotic resistance markers, these metabolite sensors can be used to report on pathway productivity, allowing high-throughput screening for pathway optimization. Future directions will focus on the application of biosensors to introduce feedback control into metabolic pathways, providing dynamic control strategies to increase the efficient use of cellular resources and pathway reliability.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.