ArticleVision research2011
Targeting of mouse guanylate cyclase 1 (Gucy2e) to Xenopus laevis rod outer segments.
Article in Vision research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Kinesin family 17 (osmotic avoidance abnormal-3) is dispensable for photoreceptor morphology and function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking.eLife · 2015Article
- Photoreceptors at a glance.Journal of cell science · 2015Review
- Distinct patterns of compartmentalization and proteolytic stability of PDE6C mutants linked to achromatopsia.Molecular and cellular neurosciences · 2015Article
- Submembrane assembly and renewal of rod photoreceptor cGMP-gated channel: insight into the actin-dependent process of outer segment morphogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- The GAFa domain of phosphodiesterase-6 contains a rod outer segment localization signal.Journal of neurochemistry · 2014Article
- Organization of cGMP sensing structures on the rod photoreceptor outer segment plasma membrane.Channels (Austin, Tex.) · 2014Article
- Insights into the role of RD3 in guanylate cyclase trafficking, photoreceptor degeneration, and Leber congenital amaurosis.Frontiers in molecular neuroscience · 2014Review
- Molecular complexes that direct rhodopsin transport to primary cilia.Progress in retinal and eye research · 2014Review
- Protein sorting, targeting and trafficking in photoreceptor cells.Progress in retinal and eye research · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Photoreceptor guanylate cyclase (GC1) is a transmembrane protein and responsible for synthesis of cGMP, the secondary messenger of phototransduction. It consists of an extracellular domain, a single transmembrane domain, and an intracellular domain. It is unknown how GC1 targets to the outer segments where it resides. To identify a putative GC1 targeting signal, we generated a series of peripheral membrane and transmembrane constructs encoding extracellular and intracellular mouse GC1 fragments fused to EGFP. The constructs were expressed in Xenopus laevis rod photoreceptors under the control of the rhodopsin promoter. We examined the localization of GFP-GC1 fusion proteins containing the complete GC1 sequence, or partial GC1 sequences, which were membrane-associated via either the GC1 transmembrane domain or the rhodopsin C-terminal palmitoyl chains. Full-length GFP-GC1 targeted to the rod outer segment disk rims. As a group, fusion proteins containing the entire cytoplasmic domain of GC1 targeted to the OS, whereas other fusion proteins containing portions of the cytoplasmic or the extracellular domains did not. We conclude that GC1 likely has no single linear peptide-based OS targeting signal. Our results suggest targeting is due to either multiple weak signals in the cytoplasmic domain of GC1, or co-transport to the OS with an accessory protein.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.