ArticleDevelopmental biology2011
Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival.
Article in Developmental biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Mettl3 Regulates Lens Development by Promoting the Differentiation Processes of Secondary Fiber Cells.Investigative ophthalmology & visual science · 2025Article
- Fibroblast growth factor-induced lens fiber cell elongation is driven by the stepwise activity of Rho and Rac.Development (Cambridge, England) · 2024Article
- The generation and characterization of a transgenic zebrafish line with lens-specific Cre expression.Molecular vision · 2024Article
- Spatially Resolved Proteomics Reveals Lens Suture-Related Cell-Cell Junctional Protein Distributions.Investigative ophthalmology & visual science · 2023Article
- Znhit1 Regulates p21Cip1 to Control Mouse Lens Differentiation.Investigative ophthalmology & visual science · 2022Article
- Drebrin, an actin-binding protein, is required for lens morphogenesis and growth.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis.iScience · 2021Article
- Calponin-3 deficiency augments contractile activity, plasticity, fibrogenic response and Yap/Taz transcriptional activation in lens epithelial cells and explants.Scientific reports · 2020Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- FHL2 mediates podocyte Rac1 activation and foot process effacement in hypertensive nephropathy.Scientific reports · 2019Article
- Ankyrin-G regulated epithelial phenotype is required for mouse lens morphogenesis and growth.Developmental biology · 2019Article
- Proteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers.Experimental eye research · 2019Article
- CB1R regulates CDK5 signaling and epigenetically controls Rac1 expression contributing to neurobehavioral abnormalities in mice postnatally exposed to ethanol.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2019Article
- Article
- Functional role for stable microtubules in lens fiber cell elongation.Experimental cell research · 2018Article
- Induction of Immune Surveillance of the Dysmorphogenic Lens.Scientific reports · 2017Article
- Signaling and Gene Regulatory Networks in Mammalian Lens Development.Trends in genetics : TIG · 2017Review
- Functional non-coding polymorphism in an EPHA2 promoter PAX2 binding site modifies expression and alters the MAPK and AKT pathways.Scientific reports · 2017Article
- N-cadherin regulates signaling mechanisms required for lens fiber cell elongation and lens morphogenesis.Developmental biology · 2017Article
- The molecular mechanisms underlying lens fiber elongation.Experimental eye research · 2017Review
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7 authors.
Funding
Abstract
Morphogenesis and shape of the ocular lens depend on epithelial cell elongation and differentiation into fiber cells, followed by the symmetric and compact organization of fiber cells within an enclosed extracellular matrix-enriched elastic capsule. The cellular mechanisms orchestrating these different events however, remain obscure. We investigated the role of the Rac1 GTPase in these processes by targeted deletion of expression using the conditional gene knockout (cKO) approach. Rac1 cKO mice were derived from two different Cre (Le-Cre and MLR-10) transgenic mice in which lens-specific Cre expression starts at embryonic day 8.75 and 10.5, respectively, in both the lens epithelium and fiber cells. The Le-Cre/Rac1 cKO mice exhibited an early-onset (E12.5) and severe lens phenotype compared to the MLR-10/Rac1 cKO (E15.5) mice. While the Le-Cre/Rac1 cKO lenses displayed delayed primary fiber cell elongation, lenses from both Rac1 cKO strains were characterized by abnormal shape, impaired secondary fiber cell migration, sutural defects and thinning of the posterior capsule which often led to rupture. Lens fiber cell N-cadherin/β-catenin/Rap1/Nectin-based cell-cell junction formation and WAVE-2/Abi-2/Nap1-regulated actin polymerization were impaired in the Rac1 deficient mice. Additionally, the Rac1 cKO lenses were characterized by a shortened epithelial sheet, reduced levels of extracellular matrix (ECM) proteins and increased apoptosis. Taken together, these data uncover the essential role of Rac1 GTPase activity in establishment and maintenance of lens shape, suture formation and capsule integrity, and in fiber cell migration, adhesion and survival, via regulation of actin cytoskeletal dynamics, cell adhesive interactions and ECM turnover.
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