ArticleJournal of reproductive immunology2011
Innate immune mediator profiles and their regulation in a novel polarized immortalized epithelial cell model derived from human endocervix.
Article in Journal of reproductive immunology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 66 citations in OpenAlex.
- Monitoring Chlamydia trachomatis Development in Polarized Endocervical Epithelial Cells.Methods in molecular biology (Clifton, N.J.) · 2027Article
- ThemBio · 2026Article
- A microphysiologic human cervical model recapitulates microbial, immune, and pathogenic properties of sexually transmitted infections.Science advances · 2026Article
- Epithelial polarity: a key player inmicroLife · 2026Review
- Evidence for a post-invasion role of themBio · 2025Article
- In vitro inhibition of the CFTR ion channel in the Macaca mulatta cervix thickens cervical mucus†.Biology of reproduction · 2025Article
- ThebioRxiv : the preprint server for biology · 2025Article
- The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection.Cell reports · 2025Article
- A multi-strategy antimicrobial discovery approach reveals new ways to treat Chlamydia.PLoS biology · 2025Article
- Antibacterial effects of coniferyl alcohol-derived dehydrogenation polymer on chlamydial infectionFrontiers in chemistry · 2025Article
- Transcriptional profiling of mucus production in rhesus macaque endocervical cells under hormonal regulation†.Biology of reproduction · 2024Article
- Seminal plasma inhibits Chlamydia trachomatis infection in vitro, and may have consequences on mucosal immunity.Scientific reports · 2024Article
- Contributions of diverse models of the female reproductive tract to the study of Chlamydia trachomatis-host interactions.Current opinion in microbiology · 2024Review
- ThemBio · 2023Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2023Article
- A 2-pyridone amide inhibitor of transcriptional activity inAntimicrobial agents and chemotherapy · 2023Article
- Hormonal regulation of non-cystic fibrosis transmembrane conductance regulator ion channels in the endocervix.F&S science · 2023Article
- Functional evaluation of the cystic fibrosis transmembrane conductance regulator in the endocervix†.Biology of reproduction · 2022Article
- Three-dimensional models of the cervicovaginal epithelia to study host-microbiome interactions and sexually transmitted infections.Pathogens and disease · 2022Article
- Mucus secretions from a conditionally reprogrammed primary endocervical cell culture.F&S science · 2022Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
The endocervix in the female reproductive tract (FRT) is susceptible to sexually transmitted pathogens such as Chlamydia trachomatis and Neisseria gonorrhoeae. Endocervical epithelial cells in vivo make innate immune mediators that likely aid in the protection from these pathogens. In vitro studies to investigate the innate epithelial cell immune response to endocervical pathogens have been hindered by the paucity of human endocervix-derived epithelial cell lines that display the differentiation proteins and functional characteristics of their site of origin. We have established an immortalized epithelial cell line (A2EN) derived from an endocervical tissue explant that can be polarized to exhibit distinct apical and basolateral membrane domains. Polarized A2EN cells secrete mucus at their apical surface, and express MUC5B, a mucin specific to the endocervix. Polarized A2EN cells also express hormone receptors that respond appropriately to female steroid hormones. Polarized A2EN cells can be stimulated with the toll-like receptor 3 agonist, polyI:C, to express anti-microbial peptides (AMPs) as well as pro-inflammatory cytokines and chemokines. Cytokines and chemokines are also differentially secreted depending on the hormone milieu in which the cells are exposed. We conclude that polarized A2EN cells maintain distinctive phenotypic and functional characteristics of the epithelial cells found in the endocervix and, hence, could provide a useful, new in vitro model system for investigations on the role of endogenous and exogenous factors that regulate endocervical epithelial cell immunity including studies on sexually transmitted infections and topical microbicides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.