ArticleBiophysical journal2011
Development of time-integrated multipoint moment analysis for spatially resolved fluctuation spectroscopy with high time resolution.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Cytoplasmic crowding acts as a porous medium reducing macromolecule diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Brain organoid protocols and limitations.Frontiers in cellular neuroscience · 2024Review
- Number and Brightness Analysis: Visualization of Protein Oligomeric State in Living Cells.Advances in experimental medicine and biology · 2021Article
- Cell volume change through water efflux impacts cell stiffness and stem cell fate.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Spatial organization of the Ran pathway by microtubules in mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching.Nature protocols · 2015Article
- Scan and conquer: a novel approach to the analysis of interactions by molecular brightness determination.Biophysical journal · 2014Article
- Time-averaged fluorescence intensity analysis in fluorescence fluctuation polarization sensitive experiments.Biomedical optics express · 2013Article
- Cellular response to heat shock studied by multiconfocal fluorescence correlation spectroscopy.Biophysical journal · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatial gradients in the behaviors of soluble proteins are thought to underlie many phenomena in cell and developmental biology, but the nature and even the existence of these gradients are often unclear because few techniques can adequately characterize them. Methods with sufficient temporal resolution to study the dynamics of diffusing molecules can only sample relatively small regions, whereas methods that are capable of imaging larger areas cannot probe fast timescales. To overcome these limitations, we developed and implemented time-integrated multipoint moment analysis (TIMMA), a form of fluorescence fluctuation spectroscopy that is capable of probing timescales down to 20 μs at hundreds of different locations simultaneously in a sample. We show that TIMMA can be used to measure the diffusion of small-molecule dyes and fluorescent colloids, and that it can create spatial maps of the behavior of soluble fluorescent proteins throughout mammalian tissue culture cells. We also demonstrate that TIMMA can characterize internal gradients in the diffusion of freely moving proteins in single cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.