ArticleBiophysical journal2011
Label-free characterization of cancer-activated fibroblasts using infrared spectroscopic imaging.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Investigating Spectral Biomarker Candidates for Migratory Potential in Cancer Cells Using Micro-FTIR and O‑PTIR Spectroscopy.ACS measurement science au · 2026Article
- Infrared spectral microscopy as a tool to monitor lung fibrosis development in a model system.Biomedical optics express · 2020Article
- An Innovative Platform Merging Elemental Analysis and Ftir Imaging for Breast Tissue Analysis.Scientific reports · 2019Article
- Label-Free Raman Spectroscopy Reveals Signatures of Radiation Resistance in the Tumor Microenvironment.Cancer research · 2019Article
- Infrared spectroscopic imaging: Label-free biochemical analysis of stroma and tissue fibrosis.The international journal of biochemistry & cell biology · 2017Review
- Emerging Themes in Image Informatics and Molecular Analysis for Digital Pathology.Annual review of biomedical engineering · 2016Review
- Development of a practical spatial-spectral analysis protocol for breast histopathology using Fourier transform infrared spectroscopic imaging.Faraday discussions · 2016Article
- Transformable Peptide Nanocarriers for Expeditious Drug Release and Effective Cancer Therapy via Cancer-Associated Fibroblast Activation.Angewandte Chemie (International ed. in English) · 2016Article
- Raman spectroscopic sensing of carbonate intercalation in breast microcalcifications at stereotactic biopsy.Scientific reports · 2015Article
- Article
- A FTIR imaging characterization of fibroblasts stimulated by various breast cancer cell lines.PloS one · 2014Article
- Article
- Quantifying collagen structure in breast biopsies using second-harmonic generation imaging.Biomedical optics express · 2012Article
- Attenuated total reflectance Fourier-transform infrared spectroscopic imaging for breast histopathology.Vibrational Spectroscopy · 2012Article
- Article
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4 authors.
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Abstract
Glandular tumors arising in epithelial cells comprise the majority of solid human cancers. Glands are supported by stroma, which is activated in the proximity of a tumor. Activated stroma is often characterized by the molecular expression of α-smooth muscle actin (α-SMA) within fibroblasts. However, the precise spatial and temporal evolution of chemical changes in fibroblasts upon epithelial tumor signaling is poorly understood. Here we report a label-free method to characterize fibroblast changes by using Fourier transform infrared spectroscopic imaging and comparing spectra with α-SMA expression in primary normal human fibroblasts. We recorded the fibroblast activation process by spectroscopic imaging using increasingly tissue-like conditions: 1), stimulation with the growth factor TGFβ1; 2), coculture with MCF-7 human breast cancerous epithelial cells in Transwell coculture; and 3), coculture with MCF-7 in three-dimensional cell culture. Finally, we compared the spectral signatures of stromal transformation with normal and malignant human breast tissue biopsies. The results indicate that this approach reveals temporally complex spectral changes and thus provides a richer assessment than simple molecular imaging based on α-SMA expression. Some changes are conserved across culture conditions and in human tissue, providing a label-free method to monitor stromal transformations.
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