ArticlePlant methods2011
Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Clathrin-Mediated Endocytosis in Plants: Historical to Modern Advances.Traffic (Copenhagen, Denmark) · 2026Review
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Endomembrane trafficking driven by microtubule growth regulates stomatal movement in Arabidopsis.Nature communications · 2024Article
- Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis.Communications biology · 2021Article
- Visualising endocytosis in plants: past, present, and future.Journal of microscopy · 2020Article
- Defining the dance: quantification and classification of endoplasmic reticulum dynamics.Journal of experimental botany · 2020Article
- The continuing arc toward phototropic enlightenment.Journal of experimental botany · 2020Article
- Dynamics and Endocytosis of Flot1 inInternational journal of molecular sciences · 2020Article
- Article
- Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells.BMC plant biology · 2018Article
- Inroads into Internalization: Five Years of Endocytic Exploration.Plant physiology · 2018Review
- Article
- Single Event Resolution of Plant Plasma Membrane Protein Endocytosis by TIRF Microscopy.Frontiers in plant science · 2017Article
- Article
- Single-Molecule Fluorescence Methods to Study Plant Hormone Signal Transduction Pathways.Frontiers in plant science · 2017Article
- Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy.Journal of visualized experiments : JoVE · 2015Article
- Multiscale imaging of plants: current approaches and challenges.Plant methods · 2015Article
- Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.Plant physiology · 2014Article
- Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis.The Plant cell · 2014Article
- Single-molecule detection and tracking in plants.Protoplasma · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTotal internal reflection fluorescence microscopy (TIRFM) is a powerful tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells. However, the utility of TIRFM in plant cell studies has been limited by the fact that plants have cell walls, thick peripheral layers surrounding the plasma membrane. Recently, a new technique known as variable-angle epifluorescence microscopy (VAEM) was developed to circumvent this problem. However, the lack of a detailed analysis of the optical principles underlying VAEM has limited its applications in plant-cell biology.
resultsHere, we present theoretical and experimental evidence supporting the use of variable-angle TIRFM in observations of intact plant cells. We show that when total internal reflection occurs at the cell wall/cytosol interface with an appropriate angle of incidence, an evanescent wave field of constant depth is produced inside the cytosol. Results of experimental TIRFM observations of the dynamic behaviors of phototropin 1 (a membrane receptor protein) and clathrin light chain (a vesicle coat protein) support our theoretical analysis.
conclusionsThese findings demonstrate that variable-angle TIRFM is appropriate for quantitative live imaging of cells in intact tissues of Arabidopsis thaliana.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.