ArticleBMC evolutionary biology2011
A bacterial genome in transition--an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus.
Article in BMC evolutionary biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 24 citations in OpenAlex.
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- Ongoing diversification of the global fish pathogen Piscirickettsia salmonis through genetic isolation and transposition bursts.The ISME journal · 2023Article
- Ecology and evolution of chlamydial symbionts of arthropods.ISME communications · 2022Article
- The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiont Amoebophilus asiaticus.FEMS microbiology ecology · 2022Article
- The Evolution of Interdependence in a Four-Way Mealybug Symbiosis.Genome biology and evolution · 2021Article
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- Comparative Analysis of theFrontiers in microbiology · 2017Article
- Babela massiliensis, a representative of a widespread bacterial phylum with unusual adaptations to parasitism in amoebae.Biology direct · 2015Article
- Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in Microcystis.The ISME journal · 2014Article
- Bacterial insertion sequences: their genomic impact and diversity.FEMS microbiology reviews · 2014Review
- The genome of Cardinium cBtQ1 provides insights into genome reduction, symbiont motility, and its settlement in Bemisia tabaci.Genome biology and evolution · 2014Article
- Evolution of small prokaryotic genomes.Frontiers in microbiology · 2014Review
- Genome degeneration and adaptation in a nascent stage of symbiosis.Genome biology and evolution · 2014Article
- Lateral transfers of insertion sequences between Wolbachia, Cardinium and Rickettsia bacterial endosymbionts.Heredity · 2013Article
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- Comparative genomics of Brachyspira pilosicoli strains: genome rearrangements, reductions and correlation of genetic compliment with phenotypic diversity.BMC genomics · 2012Article
- TnpPred: A Web Service for the Robust Prediction of Prokaryotic Transposases.Comparative and functional genomics · 2012Article
- Comparative genomics suggests an independent origin of cytoplasmic incompatibility in Cardinium hertigii.PLoS genetics · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundInsertion sequence (IS) elements are important mediators of genome plasticity and are widespread among bacterial and archaeal genomes. The 1.88 Mbp genome of the obligate intracellular amoeba symbiont Amoebophilus asiaticus contains an unusually large number of transposase genes (n = 354; 23% of all genes).
resultsThe transposase genes in the A. asiaticus genome can be assigned to 16 different IS elements termed ISCaa1 to ISCaa16, which are represented by 2 to 24 full-length copies, respectively. Despite this high IS element load, the A. asiaticus genome displays a GC skew pattern typical for most bacterial genomes, indicating that no major rearrangements have occurred recently. Additionally, the high sequence divergence of some IS elements, the high number of truncated IS element copies (n = 143), as well as the absence of direct repeats in most IS elements suggest that the IS elements of A. asiaticus are transpositionally inactive. Although we could show transcription of 13 IS elements, we did not find experimental evidence for transpositional activity, corroborating our results from sequence analyses. However, we detected contiguous transcripts between IS elements and their downstream genes at nine loci in the A. asiaticus genome, indicating that some IS elements influence the transcription of downstream genes, some of which might be important for host cell interaction.
conclusionsTaken together, the IS elements in the A. asiaticus genome are currently in the process of degradation and largely represent reflections of the evolutionary past of A. asiaticus in which its genome was shaped by their activity.
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