ArticleAnalytical chemistry2011
Micropatterned aptasensors for continuous monitoring of cytokine release from human leukocytes.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 97 citations in OpenAlex.
- The potential of microfluidic cell analysis in CAR T cell therapy manufacturing.Frontiers in bioengineering and biotechnology · 2025Review
- Perspective-Assessing Electrochemical, Aptamer-Based Sensors for Dynamic Monitoring of Cellular Signaling.ECS sensors plus · 2023Article
- Integrated Bioluminescent Immunoassays for High-Throughput Sampling and Continuous Monitoring of Cytokines.Analytical chemistry · 2023Article
- Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models.Sensors (Basel, Switzerland) · 2022Review
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- High frequency, calibration-free molecular measurementsChemical science · 2019Article
- On-chip structure-switching aptamer-modified magnetic nanobeads for the continuous monitoring of interferon-gamma ex vivo.Microsystems & nanoengineering · 2019Article
- Recent Microdevice-Based Aptamer Sensors.Micromachines · 2018Review
- A fluorescent microbead-based microfluidic immunoassay chip for immune cell cytokine secretion quantification.Lab on a chip · 2018Article
- Pilot study on an innovative biosensor with a range of medical and surgical applications.BMC research notes · 2018Article
- Article
- Calibration-Free Electrochemical Biosensors Supporting Accurate Molecular Measurements Directly in Undiluted Whole Blood.Journal of the American Chemical Society · 2017Article
- Detecting cell-secreted growth factors in microfluidic devices using bead-based biosensors.Microsystems & nanoengineering · 2017Article
- Optics-Integrated Microfluidic Platforms for Biomolecular Analyses.Biophysical journal · 2016Review
- A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip.Biosensors · 2015Article
- Label-free cytokine micro- and nano-biosensing towards personalized medicine of systemic inflammatory disorders.Advanced drug delivery reviews · 2015Review
- Detection of protein biomarker using a blood glucose meter.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Bioanalytical chemistry of cytokines--a review.Analytica chimica acta · 2015Review
- Achieving reproducible performance of electrochemical, folding aptamer-based sensors on microelectrodes: challenges and prospects.Analytical chemistry · 2014Article
- Detecting transforming growth factor-β release from liver cells using an aptasensor integrated with microfluidics.Analytical chemistry · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We report the development of a microdevice for detecting local interferon gamma (IFN-γ) release from primary human leukocytes in real time. Our microdevice makes use of miniature aptamer-modified electrodes integrated with microfluidics to monitor cellular production of IFN-γ. The aptamer species consists of a DNA hairpin molecule with thiol groups on the 3'-end for self-assembly onto Au electrodes. A redox reporter is covalently attached at the 5'-end for electrochemical sensing. This aptasensor has excellent sensitivity for IFN-γ (<60 pM detection limit) and responds to the target analyte in real time without additional washing or labeling steps. Aptamer-functionalized electrode arrays are fabricated on glass slides containing poly(ethylene glycol) (PEG) hydrogel patterns designed to expose glass regions adjacent to electrodes while protecting the remainder of the surface from nonspecific adsorption. The micropatterned substrates are integrated with PDMS microfluidic channels and incubated with T-cell-specific antibodies (Ab) (anti-CD4). Upon injection of blood, leukocytes are bound to Ab-modified glass regions in proximity to aptasensors. Cytokine release from captured cells is triggered by mitogenic activation and detected at the aptamer-modified electrodes using square wave voltammetry (SWV). The IFN-γ signal is monitored in real time with signal appearing as early as 15 min poststimulation from as few as 90 T cells. The observed IFN-γ release profiles are used to calculate an initial IFN-γ production rate of 0.0079 pg cell(-1) h(-1) upon activation. The work described here represents an important step toward development of aptasensors for immune cell analysis and blood-based diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.