ArticleJournal of water and health2011
Human norovirus infection of caco-2 cells grown as a three-dimensional tissue structure.
Article in Journal of water and health, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- A three-dimensional high throughput assay identifies novel antibacterial molecules with activity against intracellular Shigella.npj antimicrobials and resistance · 2025Article
- In Vitro Culture of Human Norovirus in the Last 20 Years.Biomedicines · 2024Review
- Current trends and new approaches for human norovirus replication in cell culture: a literature review.Archives of virology · 2024Review
- Replication of Human Norovirus in Human Intestinal Enteroids Is Affected by Fecal Sample Processing.Viruses · 2024Article
- 3D engineered tissue models for studying human-specific infectious viral diseases.Bioactive materials · 2023Review
- Bile Goes Viral.Viruses · 2021Review
- Review
- Targeted Profiling of Immunological Genes during Norovirus Replication in Human Intestinal Enteroids.Viruses · 2021Article
- Safe-in-Man Broad Spectrum Antiviral Agents.Advances in experimental medicine and biology · 2021Article
- Roles of bile acids in enteric virus replication.Animal diseases · 2021Review
- Human sapovirus propagation in human cell lines supplemented with bile acids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Viral replicons as valuable tools for drug discovery.Drug discovery today · 2020Review
- Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges.Viruses · 2019Review
- Article
- Three-Dimensional Rotating Wall Vessel-Derived Cell Culture Models for Studying Virus-Host Interactions.Viruses · 2016Review
- Three-dimensional cell culture models for investigating human viruses.Virologica Sinica · 2016Review
- Advances in norovirus biology.Cell host & microbe · 2014Review
- Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures.Archives of virology · 2014Article
- Challenges of culturing human norovirus in three-dimensional organoid intestinal cell culture models.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Human norovirus (hNoV) infectivity was studied using a three-dimensional model of large intestinal epithelium. Large intestine Caco-2 cells were grown in rotating wall vessel bioreactors for 18-21 days at 37 degrees C and then transferred to 24-well tissue culture plates where they were infected with GI.1 and GII.4 human noroviruses collected from human challenge trials and various outbreak settings, respectively. Compared with uninfected cells, transmission micrographs of norovirus-infected cells displayed evidence of shortening or total loss of apical microvilli, and vacuolization. Quantitative reverse transcription real-time PCR (qRT-PCR) indicated an approximate 2-3 log10 increase in viral RNA copies for the infected cells. A passage experiment examined both the ability for continued viral RNA and viral antigen detection. In the passaged samples 1.01x10(6) copies ml(-1) were detected by qRT-PCR. Immune electron microscopy using primary antibody to hNoV GI.1 capsids in conjunction with 6 nm gold-labelled secondary antibodies was performed on crude cellular lysates. Localization of antibody was observed in infected but not for uninfected cells. Our present findings, coupled with earlier work with the three-dimensional small intestinal INT407 model, demonstrate the utility of 3-D cell culture methods to develop infectivity assays for enteric viruses that do not readily infect mammalian cell cultures.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.