ArticleEnzyme research2011
Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective α-L-Rhamnosidase and β-D-Glucosidase Activities of Naringinase.
Article in Enzyme research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Use of Naringinase to Modify the Sensory Quality of Foods and Increase the Bioavailability of Flavonoids: A Systematic Review.Molecules (Basel, Switzerland) · 2025Pooled it
- Extracellular Enzyme Activity and Growth of Chlorella sorokiniana in Response to COCurrent microbiology · 2026Article
- Characterization of α-L-Rhamnosidase and β-D-Glucosidase Subunits of Naringinase Immobilized on a Magnetic Polysaccharide Carrier.International journal of molecular sciences · 2025Article
- Microbial diversity, metabolic properties, and biotechnological prospects of acid-sulfate soils.World journal of microbiology & biotechnology · 2025Review
- Prunin: An Emerging Anticancer Flavonoid.International journal of molecular sciences · 2025Review
- The biotechnological potential of theApplied and environmental microbiology · 2024Review
- Naringenin ameliorates Cyclophosphamide-induced nephrotoxicity in experimental model.Saudi journal of biological sciences · 2023Article
- Optimization of Naringin and Naringenin Extraction fromPharmaceutics · 2022Article
- Waste soybean frying oil for the production, extraction, and characterization of cell-wall-associated lipases from Yarrowia lipolytica.Bioprocess and biosystems engineering · 2021Article
- "Sweet Flavonoids": Glycosidase-Catalyzed Modifications.International journal of molecular sciences · 2018Review
- Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis.Scientific reports · 2017Article
- Glycosylation Is a Major Regulator of Phenylpropanoid Availability and Biological Activity in Plants.Frontiers in plant science · 2016Review
- The structure of a Streptomyces avermitilis α-L-rhamnosidase reveals a novel carbohydrate-binding module CBM67 within the six-domain arrangement.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of flavonoid glycosides by removing rhamnose from rutinosides can be accomplished through enzymatic catalysis. Naringinase is an enzyme complex, expressing both α-L-rhamnosidase and β-D-glucosidase activities, with application in glycosides hydrolysis. To produce monoglycosylated flavonoids with naringinase, the expression of β-D-glucosidase activity is not desirable leading to the need of expensive methods for α-L-rhamnosidase purification. Therefore, the main purpose of this study was the inactivation of β-D-glucosidase activity expressed by naringinase keeping α-L-rhamnosidase with a high retention activity. Response surface methodology (RSM) was used to evaluate the effects of temperature and pH on β-D-glucosidase inactivation. A selective inactivation of β-D-glucosidase activity of naringinase was achieved at 81.5°C and pH 3.9, keeping a very high residual activity of α-L-rhamnosidase (78%). This was a crucial achievement towards an easy and cheap production method of very expensive flavonoids, like prunin and isoquercetin starting from naringin and rutin, respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.