ArticleStem cells international2011
An inducible expression system of the calcium-activated potassium channel 4 to study the differential impact on embryonic stem cells.
Article in Stem cells international, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Atrial fibroblast-derived exosomal miR-21 upregulate myocardial KCa3.1 via the PI3K-Akt pathway during rapid pacing.Heliyon · 2024Article
- M2 macrophage‑derived exosomes alleviate KCa3.1 channel expression in rapidly paced HL‑1 myocytes via the NF‑κB (p65)/STAT3 signaling pathway.Molecular medicine reports · 2024Article
- Electrophysiological engineering of heart-derived cells with calcium-dependent potassium channels improves cell therapy efficacy for cardioprotection.Nature communications · 2021Article
- Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase.International journal of molecular sciences · 2021Article
- Role of intermediate-conductance calcium-activated potassium channels in atrial fibrillation in canines with rapid atrial pacing.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2021Article
- Cardiac small-conductance calcium-activated potassium channels in health and disease.Pflugers Archiv : European journal of physiology · 2021Review
- Inhibition of KCa3.1 Channels Suppresses Atrial Fibrillation via the Attenuation of Macrophage Pro-inflammatory Polarization in a Canine Model With Prolonged Rapid Atrial Pacing.Frontiers in cardiovascular medicine · 2021Article
- Adipose‑derived stem cells overexpressing SK4 calcium‑activated potassium channel generate biological pacemakers.International journal of molecular medicine · 2019Article
- SK4 KEMBO molecular medicine · 2017Article
- Mechanisms underlying the cardiac pacemaker: the role of SK4 calcium-activated potassium channels.Acta pharmacologica Sinica · 2016Review
- Re-membering the body: applications of computational neuroscience to the top-down control of regeneration of limbs and other complex organs.Integrative biology : quantitative biosciences from nano to macro · 2015Review
- A time frame permissive for Protein Kinase D2 activity to direct angiogenesis in mouse embryonic stem cells.Scientific reports · 2015Article
- Mechanism of automaticity in cardiomyocytes derived from human induced pluripotent stem cells.Journal of molecular and cellular cardiology · 2015Article
- Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo.Molecular biology of the cell · 2014Article
- SK4 Ca2+ activated K+ channel is a critical player in cardiac pacemaker derived from human embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation.Stem cells international · 2013Article
- Ca2+ activated K channels-new tools to induce cardiac commitment from pluripotent stem cells in mice and men.Stem cell reviews and reports · 2012Article
- Stem cells in heart failure.Stem cells international · 2011Article
- Reprogramming cells and tissue patterning via bioelectrical pathways: molecular mechanisms and biomedical opportunities.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Rationale. The family of calcium-activated potassium channels consists of four members with varying biological functions and conductances. Besides membrane potential modulation, SK channels have been found to be involved in cardiac pacemaker cell development from ES cells and morphological shaping of neural stem cells. Objective. Distinct SK channel subtype expression in ES cells might elucidate their precise impact during cardiac development. We chose SK channel subtype 4 as a potential candidate influencing embryonic stem cell differentiation. Methods. We generated a doxycycline inducible mouse ES cell line via targeted homologous recombination of a cassette expressing a bicistronic construct encoding SK4 and a fluorophore from the murine HPRT locus. Conclusion. We characterized the mouse ES cell line iSK4-AcGFP. The cassette is readily expressed under the control of doxycycline, and the overexpression of SK4 led to an increase in cardiac and pacemaker cell differentiation thereby serving as a unique tool to characterize the cell biological variances due to specific SK channel overexpression.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.