ArticleJournal of clinical microbiology2011
Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed.
- Early detection of pertussis related activity by wastewater surveillance ofEmerging microbes & infections · 2026Article
- Emergence of Macrolide-Resistant Bordetella pertussis, Peru, 2025.Emerging infectious diseases · 2026Article
- Clinical Profile, Outcome and Risk Factors for Mortality Among Children Admitted with Critical Pertussis: An Ambispective Study.Indian pediatrics · 2026Article
- Laboratory diagnosis of pertussis: A survey on provincial public health laboratory methods.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2026Article
- External quality assessment for yaws elimination in low- and middle-income countries using plasmid-based proficiency test items.PLoS neglected tropical diseases · 2026Article
- Comparing Respiratory Illness Surveillance Case Definitions to Detect Bordetella pertussis in Children Aged <5 Years With Respiratory Illness in South Africa, 2017-2023.The Journal of infectious diseases · 2026Article
- Positivity analysis ofMicrobiology spectrum · 2025Article
- Emergence of Bordetella holmesii-Associated Pertussis-Like Illness, Northern India, 2019-2023.Emerging infectious diseases · 2025Article
- Bordetella pertussis infection and antibody dynamics in household cohorts in two South African communities, 2016 - 2018: findings from the PHIRST study.The Journal of infection · 2025Article
- Vaccine antigen-based genotyping ofMicrobiology spectrum · 2025Article
- Draft genome sequences ofMicrobiology resource announcements · 2025Article
- Pertussis in the Post-COVID-19 Era: Resurgence, Diagnosis, and Management.Infection & chemotherapy · 2025Review
- Assessing the Impact of the 2020 Council of State and Territorial Epidemiologists Case Definition for Pertussis on Reported Pertussis Cases.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2024Article
- StrengtheningJournal of clinical microbiology · 2024Article
- Promoting molecular diagnostic equity: assessing in-house real-time PCR forLe infezioni in medicina · 2024Article
- Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis ofCurrent research in parasitology & vector-borne diseases · 2024Article
- Chlamydophila pneumoniae-associated community-acquired pneumonia in paediatric patients of a tertiary care hospital in Mexico: molecular diagnostic and clinical insights.Scientific reports · 2023Article
- Genomic characterization ofMicrobial genomics · 2023Article
- Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology.The Journal of molecular diagnostics : JMD · 2023Review
- Review
32 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel multitarget real-time PCR (RT-PCR) assay for the rapid identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using multicopy insertion sequences (ISs) in combination with the pertussis toxin subunit S1 (ptxS1) singleplex assay. The RT-PCR targets for the multiplex assay include IS481, commonly found in B. pertussis and B. holmesii; IS1001 of B. parapertussis; and the IS1001-like sequence of B. holmesii. Overall, 402 Bordetella species and 66 non-Bordetella species isolates were tested in the multitarget assay. Cross-reactivity was found only with 5 B. bronchiseptica isolates, which were positive with IS1001 of B. parapertussis. The lower limit of detection (LLOD) of the multiplex assay was similar to the LLOD of each target in an individual assay format, which was approximately 1 genomic equivalent per reaction for all targets. A total of 197 human clinical specimens obtained during cough-illness outbreak investigations were used to evaluate the multitarget RT-PCR assay. The multiplex assay results from 87 clinical specimens were compared to the individual RT-PCR assay and culture results. The multitarget assay is useful as a diagnostic tool to confirm B. pertussis infections and to rapidly identify other Bordetella species. In conclusion, the use of this multitarget RT-PCR approach increases specificity, while it decreases the amount of time, reagents, and specimen necessary for RT-PCRs used for accurate diagnosis of pertussis-like illness.
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