ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Knocking out P2X receptors reduces transmitter secretion in taste buds.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 63 citations in OpenAlex.
- Neuropeptide Y and peptide YY differentially modulate bitter- and fatty acid-evoked responses in human fungiform taste bud cells.American journal of physiology. Cell physiology · 2026Article
- Safety, Tolerability, and Pharmacokinetics of Filapixant, a Highly Selective P2X3 Receptor Antagonist, in an Ascending-Single-Dose First-in-Human Study.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Article
- Pharmacological interaction and immune response of purinergic receptors in therapeutic modulation.Purinergic signalling · 2024Review
- Constitutive and evoked release of ATP in adult mouse olfactory epithelium.Open life sciences · 2024Article
- Give-and-take of gustation: the interplay between gustatory neurons and taste buds.Chemical senses · 2024Review
- Physiology of the tongue with emphasis on taste transduction.Physiological reviews · 2023Review
- Brain-derived neurotrophic factor overexpression in taste buds diminishes chemotherapy induced taste loss.The European journal of neuroscience · 2022Article
- Sweet Taste Signaling: The Core Pathways and Regulatory Mechanisms.International journal of molecular sciences · 2022Review
- The Role of ATP and Purinergic Receptors in Taste Signaling.Handbook of experimental pharmacology · 2022Review
- "Tripartite Synapses" in Taste Buds: A Role for Type I Glial-like Taste Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- WAO-ARIA consensus on chronic cough - Part 1: Role of TRP channels in neurogenic inflammation of cough neuronal pathways.The World Allergy Organization journal · 2021Review
- Purinergic neurotransmission in the gustatory system.Autonomic neuroscience : basic & clinical · 2021Article
- Chemical and electrical synaptic interactions among taste bud cells.Current opinion in physiology · 2021Article
- Update of P2X receptor properties and their pharmacology: IUPHAR Review 30.British journal of pharmacology · 2021Review
- To Inhibit or Enhance? Is There a Benefit to Positive Allosteric Modulation of P2X Receptors?Frontiers in pharmacology · 2020Review
- Molecular mechanisms of human P2X3 receptor channel activation and modulation by divalent cation bound ATP.eLife · 2019Article
- Taste Receptors: New Players in Sperm Biology.International journal of molecular sciences · 2019Review
- Inflammation-induced upregulation of P2XAmerican journal of physiology. Lung cellular and molecular physiology · 2019Article
- Development of Full Sweet, Umami, and Bitter Taste Responsiveness Requires Regulator of G protein Signaling-21 (RGS21).Chemical senses · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
In response to gustatory stimulation, taste bud cells release a transmitter, ATP, that activates P2X2 and P2X3 receptors on gustatory afferent fibers. Taste behavior and gustatory neural responses are largely abolished in mice lacking P2X2 and P2X3 receptors [P2X2 and P2X3 double knock-out (DKO) mice]. The assumption has been that eliminating P2X2 and P2X3 receptors only removes postsynaptic targets but that transmitter secretion in mice is normal. Using functional imaging, ATP biosensor cells, and a cell-free assay for ATP, we tested this assumption. Surprisingly, although gustatory stimulation mobilizes Ca(2+) in taste Receptor (Type II) cells from DKO mice, as from wild-type (WT) mice, taste cells from DKO mice fail to release ATP when stimulated with tastants. ATP release could be elicited by depolarizing DKO Receptor cells with KCl, suggesting that ATP-release machinery remains functional in DKO taste buds. To explore the difference in ATP release across genotypes, we used reverse transcriptase (RT)-PCR, immunostaining, and histochemistry for key proteins underlying ATP secretion and degradation: Pannexin1, TRPM5, and NTPDase2 (ecto-ATPase) are indistinguishable between WT and DKO mice. The ultrastructure of contacts between taste cells and nerve fibers is also normal in the DKO mice. Finally, quantitative RT-PCR show that P2X4 and P2X7, potential modulators of ATP secretion, are similarly expressed in taste buds in WT and DKO taste buds. Importantly, we find that P2X2 is expressed in WT taste buds and appears to function as an autocrine, positive feedback signal to amplify taste-evoked ATP secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.