ArticleAquaculture (Amsterdam, Netherlands)2011
Tol2-mediated transgenesis in tilapia (Oreochromis niloticus).
Article in Aquaculture (Amsterdam, Netherlands), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Highly efficient transgenesis mediated by Tip100 transposon system in medaka.Transgenic research · 2025Article
- TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation.Cell reports methods · 2025Article
- A pheromone receptor in cichlid fish mediates attraction to females but inhibits male parental care.Current biology : CB · 2024Article
- Genome editing in East African cichlids and tilapias: state-of-the-art and future directions.Open biology · 2023Review
- East African cichlid fishes.EvoDevo · 2023Review
- Manipulation of the Tyrosinase gene permits improved CRISPR/Cas editing and neural imaging in cichlid fish.Scientific reports · 2021Article
- Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts.Scientific reports · 2019Article
- Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus) - towards understanding gene function and regulatory evolution in an ecological model system for rapid phenotypic diversification.BMC developmental biology · 2017Article
- Transposons As Tools for Functional Genomics in Vertebrate Models.Trends in genetics : TIG · 2017Review
- A compendium of developmental gene expression in Lake Malawi cichlid fishes.BMC developmental biology · 2017Article
- Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution.Transgenic research · 2016Article
- Identification of prohormones and pituitary neuropeptides in the African cichlid, Astatotilapia burtoni.BMC genomics · 2016Article
- Cichlid fishes as a model to understand normal and clinical craniofacial variation.Developmental biology · 2016Article
- Using an integrative approach to investigate the evolution of behaviour.Evolutionary applications · 2016Review
- Embryonic and larval development in the Midas cichlid fish species flock (Amphilophus spp.): a new evo-devo model for the investigation of adaptive novelties and species differences.BMC developmental biology · 2015Article
- Article
- Carotenoid-based coloration in cichlid fishes.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2014Review
- Gene transfer and mutagenesis mediated by Sleeping Beauty transposon in Nile tilapia (Oreochromis niloticus).Transgenic research · 2013Article
- Tol2-mediated generation of a transgenic haplochromine cichlid, Astatotilapia burtoni.PloS one · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Nile tilapia (Oreochromis niloticus) is an important species in aquaculture and an excellent model system for laboratory studies. Functional genetic analysis using this species has been difficult because existing methods for producing transgenics are inefficient. Here we show that the Tol2 transposon system can be used to create transgenic tilapia with high efficiency. We constructed a line that is transgenic for GFP under control of a Xenopus elongation factor 1α (EF1α) promoter. The germline transmission rate of the Tol2 construct to the first generation was about 30%, which is much higher than conventional methods. GFP expression was strong and ubiquitous in the embryos. Application of the Tol2 system for constructing transgenics in tilapia and related species will promote research in many areas, but will be especially useful for studies of evolutionary developmental biology in the cichlid fishes of East Africa.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.