ArticleShock (Augusta, Ga.)2011
Bacterial DNA induces pulmonary damage via TLR-9 through cross-talk with neutrophils.
Article in Shock (Augusta, Ga.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Cytosolic nucleic acid sensing as driver of critical illness: mechanisms and advances in therapy.Signal transduction and targeted therapy · 2025Review
- Exosome: The Regulator of the Immune System in Sepsis.Frontiers in pharmacology · 2021Review
- Identification of Cartilage Microbial DNA Signatures and Associations With Knee and Hip Osteoarthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2020Article
- Molecular mechanism whereby paraoxonase-2 regulates coagulation activation through endothelial tissue factor in rat haemorrhagic shock model.International wound journal · 2020Article
- The Central Role and Possible Mechanisms of Bacterial DNAs in Sepsis Development.Mediators of inflammation · 2020Review
- Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2019Article
- Xuebijing Injection () and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury.Chinese journal of integrative medicine · 2018Article
- Toll-Like Receptor-9 (TLR9) is Requisite for Acute Inflammatory Response and Injury Following Lung Contusion.Shock (Augusta, Ga.) · 2016Article
- CpG DNA assists the whole inactivated H9N2 influenza virus in crossing the intestinal epithelial barriers via transepithelial uptake of dendritic cell dendrites.Mucosal immunology · 2015Article
- CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa.Journal of virology · 2015Article
- Toll-like receptor 9 signaling regulates tissue factor and tissue factor pathway inhibitor expression in human endothelial cells and coagulation in mice.Critical care medicine · 2015Article
- Article
- Bacillus anthracis genomic DNA enhances lethal toxin-induced cytotoxicity through TNF-α production.BMC microbiology · 2014Article
- Real-time analysis of the effects of toxic, therapeutic and sub-therapeutic concentrations of digitoxin on lung cancer cells.Biosensors & bioelectronics · 2014Article
- Toll-like receptors and damage-associated molecular patterns: novel links between inflammation and hypertension.American journal of physiology. Heart and circulatory physiology · 2014Review
- Toll-like receptor 9 signaling delays neutrophil apoptosis by increasing transcription of Mcl-1.PloS one · 2014Article
- NADPH oxidase and Nrf2 regulate gastric aspiration-induced inflammation and acute lung injury.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Mitochondrial DAMPs increase endothelial permeability through neutrophil dependent and independent pathways.PloS one · 2013Article
- Endotracheal tube-induced sore throat pain and inflammation is coupled to the release of mitochondrial DNA.Molecular painArticle
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Bacterial DNA (bDNA) contains hypomethylated "CpG" repeats that can be recognized by Toll-like receptor 9 (TLR-9) as a pathogen-associated molecular pattern. The ability of bDNA to initiate lung injury via TLR-9 has been inferred on the basis of studies using artificial CpG DNA. But the role of authentic bDNA in lung injury is still unknown. Moreover, the mechanisms by which CpG DNA species can lead to pulmonary injury are unknown, although neutrophils (PMNs) are thought to play a key role in the genesis of septic acute lung injury. We evaluated the effects of bDNA on PMN-endothelial cell (EC) interactions thought critical for initiation of acute lung injury. Using a biocapacitance system to monitor real-time changes in endothelial permeability, we demonstrate here that bDNA causes EC permeability in a dose-dependent manner uniquely in the presence of PMNs. These permeability changes are inhibited by chloroquine, suggesting TLR-9 dependency. When PMNs were preincubated with bDNA and applied to ECs or when bDNA was applied to ECs without PMNs, no permeability changes were detected. To study the underlying mechanisms, we evaluated the effects of bDNA on PMN-EC adherence. Bacterial DNA significantly increased PMN adherence to ECs in association with upregulated adhesion molecules in both cell types. Taken together, our results strongly support the conclusion that bDNA can initiate lung injury by stimulating PMN-EC adhesive interactions predisposing to endothelial permeability. Bacterial DNA stimulation of TLR-9 appears to promote enhanced gene expression of adhesion molecules in both cell types. This leads to PMN-EC cross-talk, which is required for injury to occur.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.