ArticleJournal of virology2011
Cooperative roles of fish protein kinase containing Z-DNA binding domains and double-stranded RNA-dependent protein kinase in interferon-mediated antiviral response.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
- Surviving and Adapting to Stress: Translational Control and the Integrated Stress Response.Antioxidants & redox signaling · 2023Review
- Susceptibility and Permissivity of Zebrafish (Viruses · 2023Article
- Subversion of Programed Cell Death by Poxviruses.Current topics in microbiology and immunology · 2023Article
- Adaptive duplication and genetic diversification of protein kinase R contribute to the specificity of bat-virus interactions.Science advances · 2022Article
- LGP2 is essential for zebrafish survival through dual regulation of IFN antiviral response.iScience · 2022Article
- Promoter Binding and Nuclear Retention Features of Zebrafish IRF Family Members in IFN Response.Frontiers in immunology · 2022Article
- The emerging regulatory roles of noncoding RNAs in immune function of fish: MicroRNAs versus long noncoding RNAs.Molecular genetics and genomics : MGG · 2021Review
- Interferon regulatory factor 1 (IRF1) and anti-pathogen innate immune responses.PLoS pathogens · 2021Review
- Divergent Antiviral Mechanisms of TwoFrontiers in immunology · 2021Article
- Cytosolic Sensors for Pathogenic Viral and Bacterial Nucleic Acids in Fish.International journal of molecular sciences · 2020Review
- PKZ, a Fish-Unique eIF2α Kinase Involved in Innate Immune Response.Frontiers in immunology · 2020Review
- The Role of the Z-DNA Binding Domain in Innate Immunity and Stress Granules.Frontiers in immunology · 2020Review
- The Fish-Specific Protein Kinase (PKZ) Initiates Innate Immune Responses via IRF3- and ISGF3-Like Mediated Pathways.Frontiers in immunology · 2019Article
- Apoptosis Induction by dsRNA-Dependent Protein Kinase R (PKR) in EPC Cells via Caspase 8 and 9 Pathways.Viruses · 2018Article
- Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes.BMC genomics · 2017Article
- PKR Activation Favors Infectious Pancreatic Necrosis Virus Replication in Infected Cells.Viruses · 2016Article
- Distinct Z-DNA binding mode of a PKR-like protein kinase containing a Z-DNA binding domain (PKZ).Nucleic acids research · 2014Article
- Structural basis for Z-DNA binding and stabilization by the zebrafish Z-DNA dependent protein kinase PKZ.Nucleic acids research · 2013Article
- Article
- Lineage-specific expansion of IFIT gene family: an insight into coevolution with IFN gene family.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) inhibits protein synthesis by phosphorylating eukaryotic translation initiation factor 2α (eIF2α). In fish species, in addition to PKR, there exists a PKR-like protein kinase containing Z-DNA binding domains (PKZ). However, the antiviral role of fish PKZ and the functional relationship between fish PKZ and PKR remain unknown. Here we confirmed the coexpression of fish PKZ and PKR proteins in Carassius auratus blastula embryonic (CAB) cells and identified them as two typical interferon (IFN)-inducible eIF2α kinases, both of which displayed an ability to inhibit virus replication. Strikingly, fish IFN or all kinds of IFN stimuli activated PKZ and PKR to phosphorylated eIF2α. Overexpression of both fish kinases together conferred much more significant inhibition of virus replication than overexpression of either protein, whereas morpholino knockdown of both made fish cells more vulnerable to virus infection than knockdown of either. The antiviral ability of fish PKZ was weaker than fish PKR, which correlated with its lower ability to phosphorylate eIF2α than PKR. Moreover, the independent association of fish PKZ or PKR reveals that each of them formed homodimers and that fish PKZ phosphorylated eIF2α independently on fish PKR and vice versa. These results suggest that fish PKZ and PKR play a nonredundant but cooperative role in IFN antiviral response.
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