ArticleThe Journal of surgical research2011
Small intestinal submucosa seeded with intestinal smooth muscle cells in a rodent jejunal interposition model.
Article in The Journal of surgical research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Cellular crosstalk of fibroblast-myofibroblast transition in intestinal homeostasis and disease.Biomarker research · 2026Review
- Evidence of glucose absorption in a neoformed intestine.Updates in surgery · 2022Article
- Surgical Treatment of Short Bowel Syndrome-The Past, the Present and the Future, a Descriptive Review of the Literature.Children (Basel, Switzerland) · 2022Review
- Bioengineering and regeneration of gastrointestinal tissue: where are we now and what comes next?Expert opinion on biological therapy · 2019Review
- Bladder biomechanics and the use of scaffolds for regenerative medicine in the urinary bladder.Nature reviews. Urology · 2018Review
- Minimal modulation of the host immune response to SIS matrix implants by mesenchymal stem cells from the amniotic fluid.Hernia : the journal of hernias and abdominal wall surgery · 2017Article
- The extracellular matrix of the gastrointestinal tract: a regenerative medicine platform.Nature reviews. Gastroenterology & hepatology · 2017Review
- Bioengineering the gut: future prospects of regenerative medicine.Nature reviews. Gastroenterology & hepatology · 2016Review
- Collagen and heparan sulfate coatings differentially alter cell proliferation and attachmentTechnology · 2016Article
- Bioengineering functional human sphincteric and non-sphincteric gastrointestinal smooth muscle constructs.Methods (San Diego, Calif.) · 2016Article
- New approaches to increase intestinal length: Methods used for intestinal regeneration and bioengineering.World journal of transplantation · 2016Review
- Living scaffolds: surgical repair using scaffolds seeded with human adipose-derived stem cells.Hernia : the journal of hernias and abdominal wall surgery · 2016Article
- Successful implantation of an engineered tubular neuromuscular tissue composed of human cells and chitosan scaffold.Surgery · 2015Article
- The use of murine-derived fundic organoids in studies of gastric physiology.The Journal of physiology · 2015Article
- Influence of mesenchymal stem cells on stomach tissue engineering using small intestinal submucosa.Journal of tissue engineering and regenerative medicine · 2015Article
- Tissue engineering in the gut: developments in neuromusculature.Gastroenterology · 2014Review
- Tissue engineering and regenerative medicine as applied to the gastrointestinal tract.Current opinion in biotechnology · 2013Review
- Leveraging "raw materials" as building blocks and bioactive signals in regenerative medicine.Tissue engineering. Part B, Reviews · 2012Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSmall intestinal submucosa (SIS) is a porcine-derived, acellular, collagen-based matrix that has been tested without seeded smooth muscle cells (SMCs) for intestinal tissue engineering. We examined the expression patterns of contractile proteins of SIS with SMCs implanted in an in vivo rodent model. MATERIALS AND
methodsIntestinal SMCs were isolated from Lewis rat pups. Four-ply tubular SMCs-seeded SIS or blank SIS scaffolds were implanted in an adult rat jejunal interposition model. Recipients were sacrificed at 2, 4, and 8 wk following the implantation. The retrieved specimens were examined using antibodies against contractile proteins of SMCs.
resultsCultured intestinal SMCs expressed α-smooth muscle actin (α-SMA), calponin, and less smooth muscle myosin heavy chain (SM-MHC) in vitro. Cell-seeded SIS scaffolds contracted significantly over 8 wk of implantation but were comparable to SIS scaffolds without cell seeding. Implanted cell-seeded SIS scaffolds at 2 wk expressed extensive α-SMA, some calponin, and minimal SM-MHC. At 4 wk, α-SMA-expressing cells decreased significantly, whereas calponin or SM-MHC expressing cells were rarely detected. A small number of α-SMA-expressing cells were present at 8 wk, whereas more calponin or SM-MHC expressing cells emerged in proximity with the anastomotic interface.
conclusionsCell-seeded SIS contracted significantly after implantation, but the expressions of contractile proteins were present at the site of SIS interposition. No organized smooth muscle was formed at the site of implantation. A better scaffold design is needed to produce structured smooth muscle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.